Site-directed mutagenesis study of the antibody 2D7 which catalyzes a reaction for insertion of Cu2+ into mesoporphyrin

• Published in: Journal of bioscience and bioengineering Vol. 99; no. 3; pp. 222 - 229
• Main Authors: Hosomi, N. (Kitasato Univ., Tokyo (Japan)), Kawamura Konishi, Y, Kawano, R, Fujii, I, Suzuki, H
• Format: Journal Article
• Language: English
• Published: Amsterdarm Elsevier Science 01.03.2005; Elsevier Limited
• Subjects: ANTIBODIES; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - immunology; Binding Sites; Biological and medical sciences; Biotechnology; Catalysis; CATALYTIC ACTIVITY; Copper - chemistry; FLUORESCENCE; Fundamental and applied biological sciences. Psychology; Immunoglobulin Fab Fragments - chemistry; Immunoglobulin Fab Fragments - genetics; Immunoglobulin Fab Fragments - immunology; Mesoporphyrins - chemistry; METALS; Mutagenesis, Site-Directed; PORPHYRINS; Protein Binding; Protein Engineering - methods; Structure-Activity Relationship; site-directed mutagenesis; Abzyme; catalytic antibody; Porphyrin; Stabilization; transition-state stabilization; Site directed mutagenesis; fluorescence quenching; Fluorescence; Metalation
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1263/jbb.99.222

Monoclonal antibody 2D7 generated against a transition-state analog N-methyl mesoporphyrin catalyzes a reaction for insertion of a cupric ion into mesoporphyrin. To investigate amino acid residues responsible for the catalytic activity, site-directed mutagenesis of the amino acid residues in the third complementarity determining region of the heavy chain (CDRH3) was performed on the antigen-binding fragment (Fab) of the antibody. Recombinant Fab mutants, in which Arg95 is replaced with Ala (R95A), Asp96 with Asn (D96N) and Met97 with Gly (M97G), were examined in terms of the catalytic efficiency of the reaction (k/Ksub(s)) and the dissociation constant for N-methyl mesoporphyrin binding (Ksub(d)) and these values were compared with those of the wild type. The k/Ksub(s) values of the R95A and D96N mutants were 0.96% and 1.0% of that of the wild type, respectively, whereas the M97G mutant had no detectable catalytic activity. The Ksub(d) values of the R95A and D96N mutants were 165 and 69 times that of the wild type, respectively, while that of the M97G mutant was similar to that of the wild type. The relationship between the k/Ksub(s) and 1/Ksub(d) values in the wild type and the R95A and D96N mutants suggests that Arg95 and Asp96 are responsible for stabilizing the transition-state in the catalytic reaction. The results of the M97G mutant allow us to propose that Met97 plays an important role in the catalytic activity probably due to a subtle and specific conformation of the antibody.