Litorilinea aerophila gen. nov., sp. nov., an aerobic member of the class Caldilineae, phylum Chloroflexi, isolated from an intertidal hot spring

• Published in: International journal of systematic and evolutionary microbiology Vol. 63; no. Pt 3; pp. 1149 - 1154
• Main Authors: Kale, Varsha, Björnsdóttir, Snædís H, Friðjónsson, Ólafur H, Pétursdóttir, Sólveig K, Ómarsdóttir, Sesselja, Hreggviðsson, Guðmundur Óli
• Format: Journal Article
• Language: English
• Published: England International Union of Microbiological Societies 01.03.2013
• Subjects: bacteria; Bacterial Typing Techniques; Base Composition; carbohydrates; Chloroflexi - classification; Chloroflexi - genetics; Chloroflexi - isolation & purification; DNA, Bacterial - genetics; Fatty Acids - analysis; hot springs; Hot Springs - microbiology; Iceland; Molecular Sequence Data; new genus; nucleotide sequences; Phospholipids - analysis; Phylogeny; Quinones - analysis; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; sodium chloride; temperature; Iceland
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.044115-0

A thermophilic, aerobic, Gram-stain-negative, filamentous bacterium, strain PRI-4131T, was isolated from an intertidal hot spring in Isafjardardjup, NW Iceland. The strain grew chemo-organotrophically on various carbohydrates. The temperature range for growth was 40–65 °C (optimum 55 °C), the pH range was pH 6.5–9.0 (optimum pH 7.0) and the NaCl range was 0–3 % (w/v) (optimum 0.5 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PRI-4131T represented a distinct lineage within the class Caldilineae of the phylum http://dx.doi.org/10.1601/nm.550Chloroflexi. The highest levels of sequence similarity, about 91 %, were with Caldilinea aerophila STL-6-O1T and Caldilinea tarbellica D1-25-10-4T. Fermentative growth was not observed for strain PRI-4131T, which, in addition to other characteristics, distinguished it from the two Caldilinea species. Owing to both phylogenetic and phenotypic differences from the described members of the class Caldilineae, we propose to accommodate strain PRI-4131T in a novel species in a new genus, Litorilinea aerophila gen. nov., sp. nov. The type strain of Litorilinea aerophila is PRI-4131T ( = DSM 25763T = ATCC BAA-2444T).