Potassium and zinc-induced frost tolerance in pistachio flowers is associated with physiological and biochemical changes

• Published in: Trees (Berlin, West) Vol. 34; no. 4; pp. 1021 - 1032
• Main Authors: Norozi, Mohammadmehdi, ValizadehKaji, Babak, Karimi, Rouhollah, Solgi, Mousa
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2020; Springer Nature B.V
• Subjects: Agriculture; Antioxidants; Biomedical and Life Sciences; Carbohydrates; Catalase; Cold regions; Cold tolerance; Cultivation; Enzymatic activity; Enzyme activity; Flowers; Forestry; Freezing; Frost; Frost damage; Frost prevention; Fruit cultivation; Fruit trees; Guaiacol; Hydrogen peroxide; Ice; Life Sciences; Lipid peroxidation; Lipids; Low temperature; Low temperature tests; Malondialdehyde; Nutrients; Original Article; Peroxidase; Peroxidation; Phenolic compounds; Phenols; Physiology; Plant Anatomy/Development; Plant Pathology; Plant Physiology; Plant Sciences; Potassium; Potassium sulfate; Proline; Proteins; Spraying; Trees; Zinc; Zinc sulfate; Freezing tolerance; Blossom; Spring frost; Mineral nutrients
• ISSN: 0931-1890; 1432-2285
• DOI: 10.1007/s00468-020-01978-9

Key message K/Zn application by spraying at bud swell stage and green tip stage improved freezing tolerance of pistachio flowers due to increased antioxidant enzymes, soluble proteins, soluble carbohydrates, total phenolic and proline contents as well as decreased lipid peroxidation and H 2 O 2 content in flower. Spring frost injury to blossoms has been a severe problem in pistachio-harvesting lands of Iran. Use of mineral nutrients has been offered as a measure to alleviate or prevent frost harm. The objective of the present study was to investigate the effects of potassium and zinc application on the spring frost damage of pistachio flowers. Treatments included application of three levels of K 2 SO 4 (0, 1, and 2%) combined with three levels of ZnSO 4 (0, 0.5, and 1%). The mineral solutions were sprayed on trees two times (bud swell stage and green tip stage) in 2017–2018. Excised 1-year-old twigs were collected during full blooming. The twigs were exposed to three different low-temperature tests (0, − 2, and − 4 °C), and physiological and biochemical changes were evaluated. The most concentrated mineral solutions, especially, 1% K 2 SO 4  + 1% ZnSO 4 , increased significantly freezing tolerance, the content of soluble proteins, soluble carbohydrates, total phenolic compounds, proline, guaiacol peroxidase, and catalase enzyme activity of flowers. Compared with control, the application of K 2 SO 4 in combination with ZnSO 4 decreased hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) concentrations of flowers after freezing tests. The results of the present study could have implications for improving the freezing tolerance of temperate fruit trees cultivated in cold regions by the use of potassium and zinc.