Soil and microbial biomass stoichiometry regulate soil organic carbon and nitrogen mineralization in rice-wheat rotation subjected to long-term fertilization

• Published in: Journal of soils and sediments Vol. 20; no. 8; pp. 3103 - 3113
• Main Authors: Ashraf, Muhammad Nadeem, Hu, Cheng, Wu, Lei, Duan, Yinghua, Zhang, Wenju, Aziz, Tariq, Cai, Andong, Abrar, Muhammad Mohsin, Xu, Minggang
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2020; Springer Nature B.V
• Subjects: Agricultural ecosystems; Agricultural practices; Availability; Biological fertilization; Biomass; Carbon dioxide; Crop rotation; Earth and Environmental Science; Environment; Environmental Physics; Farmyard manure; Fertilization; Fertilizers; Leucine; Manganese; Manures; Microorganisms; Mineral fertilizers; Mineral nutrients; Mineralization; Nitrogen; Nutrient availability; Nutrients; Organic carbon; Organic soils; Peptidase; Phosphorus; Potassium; Quotients; Rice; Rotation; Sec 1 • Soil Organic Matter Dynamics and Nutrient Cycling • Research Article; Soil; Soil nutrients; Soil Science & Conservation; Soils; Stoichiometry; Wheat; Nitrogen mineralization; Long-term fertilization; Stoichiometry; SOC mineralization; Nutrient availability; Microbial biomass
• ISSN: 1439-0108; 1614-7480
• DOI: 10.1007/s11368-020-02642-y

Purpose Soil microbial biomass (SMB), as the source and sink of soil nutrients, and its stoichiometry play a key role in soil organic carbon (SOC) and nitrogen (N) mineralization. The objective of this study was to investigate the responses of SOC and N mineralization to changes in microbial biomass and SOC, N, and phosphorus (P) stoichiometry resulted from long-term fertilization regimes. Materials and methods Soil was sampled from a rice-wheat rotation system subjected to 37 years of nine fertilization treatments with different nutrient input amounts: control (CK), N alone, N combined with mineral phosphorus (NP), NP plus potassium (NPK), manure alone (M), and M combined with N (MN), NP (MNP), NPK (MNPK), and a higher rate of M with NPK (hMNPK). The sampled soil was incubated for the determination of SOC and N mineralization, C, N, and P stoichiometry of soil and SMB, and associated soil enzymes related to C and N cycling. Results and discussion Relative to the CK and treatments with mineral fertilizers, treatments with manure (M, MN, MNP, MNPK, and hMNPK) significantly increased SOC and N mineralization by 48–78% and 54–97%, respectively. Microbial metabolic quotient ( q CO 2 ) decreased by 32–55% in treatments with manure compared to the N and NP treatments, but showed no effect on the q CO 2 when compared to the NPK treatment. The leucine amino peptidase (LAP) enzyme showed significant positive correlation with SOC and N mineralization, and negatively related to the q CO 2 . Significantly negative correlations were also observed between SOC and N mineralization and soil C:P and N:P ratio, as well as microbial biomass SMBC:SMBP and SMBN:SMBP stoichiometry, respectively. However, the availability of N and P had limited effects on the q CO 2 after reaching a certain value (0.69–0.72 mg CO 2 -C g −1  MBC h −1 ). Conclusions Lower soil elemental (C:P and N:P) and microbial biomass stoichiometry (SMBC:SMBP and SMBN:SMBP) and increase of LAP resulted from combined application of manure and mineral fertilizers, accelerated SOC, and N mineralization. Mineral nutrient input with manure amendments could be an optimal strategy to meet the microbial stoichiometric demands and enhance nutrient availability for crops in agricultural ecosystems.