UPLC-DAD determination of taxodione content in hairy roots of Salvia austriaca Jacq

• Published in: Acta chromatographica Vol. 26; no. 4; pp. 671 - 681
• Main Authors: KuZma, L, Wysokinska, H
• Format: Journal Article
• Language: English
• Published: Akademiai Kiado 01.12.2014
• Subjects: Chemical properties; Composition; High performance liquid chromatography; Identification and classification; Methods; Physiological aspects; Roots (Botany); Salvia; Terpenoids
• ISSN: 1233-2356; 2083-5736
• DOI: 10.1556/AChrom.26.2014.4.9

Taxodione is the abietane diterpenoid, which has been earlier reported to show cytotoxic, antitumor, antibacterial, and antioxidant properties. This compound has been isolated from a genetically transformed root culture of Salvia austriaca. However, no method for taxodione quantification is yet available. A validated ultra-performance liquid chromatography-diode array detector (UPLC-DAD) method was developed for the quantification of taxodione in a hexane extract taken from genetically transformed roots of S. austriaca. The developed UPLC-DAD method demonstrated good linearity in the range 10-500 [mu]g mL super(-1)(r super(2)= 0.99988), high precision (relative standard deviation (RSD) <1.42%), and good repeatability (RSD <1.84%). The limits of detection and quantification of taxodione were found to be 0.48 ng and 1.60 ng, respectively. The recovery of taxodione ranged between 90.4 and 113.1%, and the RSD was less than 2.5%.The developed UPLC-DAD method was then used to determine the content of taxodione in various hairy root lines and changes in amount of the compound during the 50-day growth cycle of the culture. The content of taxodione in hairy root cultures of S. austriaca ranged from 0.29 to 1.12 mg g super(-1) dry weight depending on root line and stage of growth.