Immunoturbidimetric determination of C-reactive protein (CRP) and high-sensitivity CRP on heparin plasma. Comparison with serum determination

• Published in: Clinical chemistry and laboratory medicine Vol. 41; no. 7; pp. 948 - 949
• Main Authors: DUPUY, Anne Marie, BADIOU, Stéphanie, DESCOMPS, Bernard, CRISTOL, Jean Paul
• Format: Journal Article
• Language: English
• Published: Berlin Walter de Gruyter 01.07.2003; New York, NY
• Subjects: Biological and medical sciences; Blood Chemical Analysis - methods; C-Reactive Protein - analysis; Cardiovascular system; Heparin; Humans; Immunoassay - methods; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Nephelometry and Turbidimetry - methods; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Reproducibility of Results; Sensitivity and Specificity; Human; Biological marker; Cardiovascular disease; Inflammation; Blood plasma; Vascular disease; High sensitivity; Measurement technique; Clinical biology; Atherosclerosis; Risk factor; Reagents; Heparin; C reactive protein
• ISSN: 1434-6621; 1437-4331
• DOI: 10.1515/CCLM.2003.144

Determination of C-reactive protein (CRP) and high-sensitivity CRP (hs-CRP), markers of systemic inflammation and atherosclerosis risk, usually requires serum samples, while heparin plasma samples are routinely collected for routine chemistry. This study evaluates the use of heparin plasma samples for hs-CRP and CRP determinations using immunoturbidimetric methods on an Olympus system. As a result, analytical performance, including linearity, imprecision values, and determination from 185 clinical samples, are not affected by the type of sampling, validating the use of heparin plasma samples for CRP and hs-CRP determination with Olympus reagents.