Extracellular lactate as an alternative energy source for retinal bipolar cells

Retinal bipolar and amacrine cells receive visual information from photoreceptors and participate in the first steps of image processing in the retina. Several studies have suggested the operation of aerobic glycolysis and a lactate shuttle system in the retina due to the high production of this met...

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Bibliographic Details
Published inThe Journal of biological chemistry Vol. 300; no. 4; p. 106794
Main Authors Calbiague-Garcia, Victor, Chen, Yiyi, Cádiz, Bárbara, Tapia, Felipe, Paquet-Durand, François, Schmachtenberg, Oliver
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.04.2024
American Society for Biochemistry and Molecular Biology
Subjects
Animals
bipolar cells
Energy Metabolism
Glucose - metabolism
lactate
lactate shuttle
Lactic Acid - metabolism
Mice
monocarboxylate transporters
Monocarboxylic Acid Transporters - genetics
Monocarboxylic Acid Transporters - metabolism
retina
Retina - cytology
Retina - metabolism
Retinal Bipolar Cells - metabolism
BC
RT
INL
RPE
Iout
LDH-A
AUC
RBC
retina
monocarboxylate transporters
MC
lactate shuttle
Δ6
AC
mTFP
PKM2
Ica
bipolar cells
DAPI
Syn
ROI
Vmemb
GFAP
ERG
lactate
MCT
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Summary:Retinal bipolar and amacrine cells receive visual information from photoreceptors and participate in the first steps of image processing in the retina. Several studies have suggested the operation of aerobic glycolysis and a lactate shuttle system in the retina due to the high production of this metabolite under aerobic conditions. However, whether bipolar cells form part of this metabolic circuit remains unclear. Here, we show that the monocarboxylate transporter 2 is expressed and functional in inner retinal neurons. Additionally, we used genetically encoded FRET nanosensors to demonstrate the ability of inner retinal neurons to consume extracellular lactate as an alternative to glucose. In rod bipolar cells, lactate consumption allowed cells to maintain the homeostasis of ions and electrical responses. We also found that lactate synthesis and transporter inhibition caused functional alterations and an increased rate of cell death. Overall, our data shed light on a notable but still poorly understood aspect of retinal metabolism.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0021-9258
1083-351X
DOI:10.1016/j.jbc.2024.106794