Rocking‐curve imaging of high‐quality protein crystals by non‐dispersive X‐ray optics in the double‐crystal configuration

The double‐crystal configuration is useful for the evaluation of strain and defects in single crystals. In this study, rocking curve measurements by X‐ray topography in the double‐crystal configuration were demonstrated using perfect crystals of the protein glucose isomerase (GI). The setup enables...

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Bibliographic Details
Published inJournal of applied crystallography Vol. 55; no. 5; pp. 1111 - 1115
Main Authors Suzuki, Ryo, Abe, Marina, Hirano, Keiichi, Kojima, Kenichi, Tachibana, Masaru
Format Journal Article
LanguageEnglish
Published 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.10.2022
Blackwell Publishing Ltd
Subjects
analyser crystals
Configurations
Crystal defects
Crystals
double‐crystal configuration
Evaluation
Geometrical optics
Glucose isomerase
high‐quality crystals
Optics
protein crystals
Proteins
rocking curve imaging
Single crystals
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Summary:The double‐crystal configuration is useful for the evaluation of strain and defects in single crystals. In this study, rocking curve measurements by X‐ray topography in the double‐crystal configuration were demonstrated using perfect crystals of the protein glucose isomerase (GI). The setup enables precise evaluation of perfection in protein crystals with the nearly nondispersive X‐ray optics. It reveals the uniform perfection of GI crystals according to the theory of X‐ray diffraction. It is expected that unknown imperfections in various protein crystals of lower quality will be revealed by the nondispersive configuration using perfect protein crystals. Rocking curve imaging using a double‐crystal configuration with perfect protein crystals is demonstrated.
ISSN:1600-5767
0021-8898
1600-5767
DOI:10.1107/S1600576722007130