Demonstration of angiogenin in human endometrium and its enhanced expression in endometrial tissues in the secretory phase and the decidua

• Published in: The journal of clinical endocrinology and metabolism Vol. 86; no. 11; pp. 5609 - 5614
• Main Authors: KOGA, Kaori, OSUGA, Yutaka, FUJIWARA, Toshihiro, TAKETANI, Yuji, TSUTSUMI, Osamu, YANO, Tetsu, YOSHINO, Osamu, TAKAI, Yasushi, MATSUMI, Hirotaka, HIROI, Hisahiko, KUGU, Koji, MOMOEDA, Mikio
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01.11.2001
• Subjects: Biological and medical sciences; Blotting, Western; Cyclic AMP - pharmacology; Decidua - metabolism; Endometrium - metabolism; Epithelial Cells - metabolism; Female; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; Humans; Hypoxia - metabolism; Immunohistochemistry; In Vitro Techniques; Mammalian female genital system; Menstrual Cycle - metabolism; Pregnancy. Parturition. Lactation; Reverse Transcriptase Polymerase Chain Reaction; Ribonuclease, Pancreatic - biosynthesis; Ribonuclease, Pancreatic - metabolism; RNA, Messenger - biosynthesis; Stromal Cells - metabolism; Vertebrates: reproduction; Human; Immunohistochemistry; Menstrual cycle; Angiogenic factor; Decidua; Gene expression; Ovarian hormone; Progestagen; Pregnancy; Pathology; Angiogenesis; Hormonal regulation; Female; Physiology; Progesterone; Sex steroid hormone; Endometrium
• ISSN: 0021-972X; 1945-7197
• DOI: 10.1210/jc.86.11.5609

Angiogenesis is thought to be crucial for normal physiology of the endometrium, where dynamic vascular remodeling occurs during the menstrual cycle and pregnancy. We investigated the presence of angiogenin, a potent inducer of angiogenesis, and the regulatory mechanisms of its production in the human endometrium. Western blot analysis demonstrated that angiogenin protein expression increased by 3- to 4-fold in the endometrium in the mid and late secretory phases and in early gestation relative to that during the proliferative phase. Quantitative mRNA analysis showed the similar tendency in the expression of angiogenin mRNA in the endometrium, with the highest levels observed in the mid and late secretory phases and early gestation. An immunohistochemical study showed that angiogenin was expressed in both stromal cells and epithelial cells, with indistinguishable intensity between these cells regardless of phases of the menstrual cycle. In support of the Western blot analysis, the intensity of staining appeared to be highest in the mid to late secretory phases relative to other phases. Consistent with these in vivo results, decidualized cultured stromal cells, after treatment with progesterone or progesterone plus E2, exhibited the capacity to secrete significantly increased amounts of angiogenin compared with untreated or E2 alone-treated control group. Both the treatment with (Bu)2cAMP and hypoxic conditions stimulated angiogenin secretion by stromal cells. For isolated epithelial cells, hypoxia stimulated angiogenin secretion, whereas (Bu)2cAMP had no appreciable effect. In summary, we demonstrated the presence of angiogenin in human endometrium and its possible local regulatory factors, such as progesterone, cAMP, and hypoxia. These findings along with its enhanced expression in the endometrium in the secretory phase and in decidual tissues raise the possibility that angiogenin may play a role in establishing pregnancy.