UVA generated free radicals in irinotecan (CPT-11) in the presence of copper ions

Irinotecan (CPT-11) is an efficient anticancer drug approved for clinical use in the treatment of advanced colorectal cancers. This work is focused on the study of photoinduced processes in irinotecan (CPT-11) both in the presence and absence of Cu(II) ions by means of UV/vis and EPR spectroscopy. S...

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Published inJournal of photochemistry and photobiology. A, Chemistry. Vol. 290; pp. 125 - 138
Main Authors Bobeničová, Miroslava, Valko, Marián, Brezová, Vlasta, Dvoranová, Dana
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.09.2014
Subjects
CPT-11
EPR spectroscopy
Spin trapping technique
Superoxide radical anion
UV/vis spectroscopy
Superoxide radical anion
CPT-11
DMSO
NS
CPT
EPR
UV/vis spectroscopy
MNP
EPR spectroscopy
Spin trapping technique
DIPPMPO
SN-38
DPPH
SW
DMPO
SOD
ACN
UV/vis
hfcc
POBN
DNA
ND
TEMPOL
TMP
ROS
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Summary:Irinotecan (CPT-11) is an efficient anticancer drug approved for clinical use in the treatment of advanced colorectal cancers. This work is focused on the study of photoinduced processes in irinotecan (CPT-11) both in the presence and absence of Cu(II) ions by means of UV/vis and EPR spectroscopy. Solid-state EPR revealed the solvent dependent coordination between CPT-11 and cupric ions. EPR spin trapping technique, using various spin trapping agents, was applied to monitor the generation of reactive radical species (oxygen- and carbon-centered radicals) in irradiated CPT-11 and CPT-11/CuCl2 systems under the given experimental conditions. We confirmed that the presence of CPT-11 is necessary for the formation of reactive radicals and the photoinduced processes are influenced by the presence of cupric ions. ROS generated upon irradiation of CPT-11 in the presence/absence of copper ions can be regarded as complementary species to the original inhibitory action of the drug on the DNA topoisomerase I.
ISSN:1010-6030
1873-2666
DOI:10.1016/j.jphotochem.2014.06.012