Archaeal promoter-directed expression of the Halobacterium salinarum catalase-peroxidase gene

• Published in: Extremophiles : life under extreme conditions Vol. 4; no. 6; pp. 351 - 356
• Main Authors: SHINONG LONG, SALIN, Marvin L
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.12.2000; Tokyo
• Subjects: Bacteriology; Base Sequence; Biological and medical sciences; Catalase - biosynthesis; Catalase - genetics; DNA Primers; Enzyme Induction; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Archaeal - genetics; Genetics; Halobacterium - enzymology; Halobacterium - genetics; Microbiology; Oxidative Stress; Peroxidases - biosynthesis; Peroxidases - genetics; Promoter Regions, Genetic; Space life sciences; Archaeobacteria; Enzyme; Transcription promoter; Mixed function enzyme; Gene expression; Stress; Halobacterium salinarum; Peroxidases; Catalase; Halophily; Halobacteriales; Bacteria; Peroxidase; Oxidoreductases; Halobacteriaceae
• ISSN: 1431-0651; 1433-4909
• DOI: 10.1007/s007920070004

The Halobacterium salinarum catalase-peroxidase gene was subcloned into shuttle vectors pWL102 and pWL202 and expressed under the control of different archaeal promoters. When Hbt. salinarum was transformed with the catalase-peroxidase gene under the control of its own promoter, catalase-peroxidase activity increased twofold. Catalase-peroxidase activity increased threefold when Hbt. salinarum was transformed with the catalase-peroxidase gene under the control of a tRNA promoter. This bifunctional enzyme in Hbt. salinarum was not induced by environmental stresses such as H2O2, intense light, darkness, high temperature, low temperature, redox inhibitors, heavy metals, or ions.