Antimicrobial resistance and subtyping of Salmonella enterica subspecies enterica serovar Enteritidis isolated from human outbreaks and poultry in southern Brazil

• Published in: Poultry science Vol. 89; no. 7; pp. 1530 - 1536
• Main Authors: Vaz, C.S.L, Streck, A.F, Michael, G.B, Marks, F.S, Rodrigues, D.P, dos Reis, E.M.F, Cardoso, M.R.I, Canal, C.W
• Format: Journal Article
• Language: English
• Published: Oxford, UK Poultry Science Association 01.07.2010; Oxford University Press
• Subjects: animal pathogenic bacteria; Animals; Anti-Bacterial Agents - pharmacology; antibiotic resistance; Bacteriophage Typing; Brazil - epidemiology; clones; disease outbreaks; Disease Outbreaks - veterinary; disease surveillance; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Drug Resistance, Multiple, Bacterial; food contamination; food microbiology; food pathogens; foodborne illness; genotype; Humans; microbial contamination; Microbial Sensitivity Tests; Polymerase Chain Reaction - veterinary; Polymorphism, Restriction Fragment Length; poultry; Poultry - microbiology; Salmonella enteritidis; Salmonella enteritidis - drug effects; Salmonella enteritidis - genetics; Salmonella enteritidis - isolation & purification; Salmonella Infections - drug therapy; Salmonella Infections - epidemiology; Salmonella Infections - microbiology; Salmonella Infections, Animal - drug therapy; Salmonella Infections, Animal - epidemiology; Salmonella Infections, Animal - microbiology; salmonellosis; strain differences; Brazil; poultry; Enteritidis; pulsed-field gel electrophoresis; antimicrobial resistance; phage type
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps.2009-00453

To investigate antimicrobial resistance, 96 Salmonella enterica subspecies enterica serovar Enteritidis strains isolated from salmonellosis outbreaks and poultry-related products obtained in southern Brazil were analyzed. Macrorestriction patterns, obtained by pulsed-field gel electrophoresis and phage types, were assessed. Although 43.75% of samples were sensitive to all drugs tested, resistance to sulfonamide (34.37%), trimethoprim-sulfamethoxazole (25.00%), nalidixic acid (14.58%), streptomycin (2.08%), gentamicin, and tetracycline (1.04%) was identified. Furthermore, 89.60% of strains belonged to phage type 4, and a predominant pulsed-field gel electrophoresis genotype represented by 82.29% of the strains was identified, suggesting that a clonal group was distributed in poultry, food, and human isolates. Although it was not possible to associate strains from different sources, the occurrence of antimicrobial-resistant Salmonella Enteritidis strains supports the need to establish monitoring programs to identify the emergence of potential resistance patterns and to direct policies for use of these drugs in food-producing animals.