Development of an Absorbance Detection Module Using a Deep UV Light-Emitting Diode for a Portable Miniaturized Liquid Chromatograph

In recent years, there has been increasing demand for miniaturized analytical instruments that allow immediate on-site analysis. We have been developing a portable HPLC system consisting of a column-integrated chip and a compact, low-power electroosmotic flow pump. This study aims to extend the vers...

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Bibliographic Details
Published inBunseki kagaku Vol. 72; no. 3; pp. 125 - 131
Main Authors KOYAMA, Kaito, NISHINURA, Takuma, ISHIDA, Akihiko, HIBINO, Mitsue, MAEKI, Masatoshi, TANI, Hirofumi, TOKESHI, Manabu
Format Journal Article
LanguageEnglish
Japanese
Published Tokyo Japan Science and Technology Agency 01.01.2023
Subjects
Absorbance
Electroosmosis
High performance liquid chromatography
Light emitting diodes
Light sources
Modules
Photodiodes
Portability
Ultraviolet radiation
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Summary:In recent years, there has been increasing demand for miniaturized analytical instruments that allow immediate on-site analysis. We have been developing a portable HPLC system consisting of a column-integrated chip and a compact, low-power electroosmotic flow pump. This study aims to extend the versatility of the portable HPLC system with a further reduction of the overall system size, and thus we constructed a UV absorbance detection module that can be mounted to the column-integrated chip. For this detection module, a UV light-emitting diode (peak wavelength = 255 nm) and photodiode were used as a light source and photodetector, and a system to control their operation and process the signals was also constructed. The developed detection module exhibited excellent performances as an HPLC detector.
ISSN:0525-1931
DOI:10.2116/bunsekikagaku.72.125