Simple and efficient synthesis of copper(II)-modified uniform magnetic Fe3O4@SiO2 core/shell microspheres for immobilization of cellulase

• Published in: Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology Vol. 15; no. 11
• Main Authors: Li, Shi-Kuo, Hou, Xiao-Cheng, Huang, Fang-Zhi, Li, Chuan-Hao, Kang, Wen-Juan, Xie, An-Jian, Shen, Yu-Hua
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 2013; Springer
• Subjects: Catalytic methods; Characterization and Evaluation of Materials; Chemistry and Materials Science; Cross-disciplinary physics: materials science; rheology; Exact sciences and technology; Growth from solutions; Inorganic Chemistry; Lasers; Materials Science; Methods of crystal growth; physics of crystal growth; Methods of nanofabrication; Nanotechnology; Optical Devices; Optics; Photonics; Physical Chemistry; Physics; Research Paper; Immobilization; Superparamagnetism; Core/shell microspheres; Cellulase; Silanes; Enzymes; Catalytic reaction; Core shell structure; Solvothermal synthesis; Crystal growth from solutions; Copper; Sol-gel process
• ISSN: 1388-0764; 1572-896X
• DOI: 10.1007/s11051-013-2013-7

In this paper, we reported a simple and efficient protocol for preparation of Cu 2+ -modified magnetic Fe 3 O 4 @SiO 2 core/shell microspheres for immobilization of cellulase. The uniform magnetic Fe 3 O 4 @SiO 2 core/shell microspheres with a thin shell of 20 nm were synthesized through a solvothermal method followed by a sol–gel process. An amino-terminated silane coupling agent of (3-aminopropyl)triethoxysilane (APTS) was then grafted on them for capturing Cu 2+ ions. The reaction process is very simple, efficient, and economical. Noticeably, the content of Cu 2+ ions on the magnetic core/shell microspheres can reach 4.6 Wt%, endowing them possess as high immobilization capacity as 225.5 mg/g for cellulase. And the immobilized cellulase can be retained over 90 % on the magnetic microspheres after six cycles. Meanwhile, the magnetic microspheres decorated with Cu 2+ ions show a superparamagnetic character with a high magnetic saturation of 58.5 emu/g at room temperature, suggesting conveniently and rapidly recycle the enzyme from solution. This facile, recyclable, high immobilization capacity and activity strategy may find potential applications in enzyme catalytic reactions with low cost.