Protein analysis on pistils and pollens of almonds [Prunus amygdalus] with special reference to Sf, a self-fertile gene

• Published in: Engei Gakkai zasshi Vol. 56; no. 3; pp. 300 - 305
• Main Authors: Yamashita, K. (Miyazaki Univ. (Japan). Faculty of Agriculture), Gaude, T, Dumas, C, Grasselly, C, Raynaud, P.C
• Format: Journal Article
• Language: English
• Published: THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE 1987
• Subjects: ANALISIS QUIMICO; ANALYSE CHIMIQUE; AUTOPOLINIZACION; AUTOPOLLINISATION; CHEMICAL ANALYSIS; ESTERILIDAD; FERTILIDAD; FERTILITE; FERTILITY; FLEUR; FLORES; FLOWERS; GENETIC VARIATION; GINECEO; GYNECEE; GYNOECIUM; INFERTILITE; INFERTILITY; POLEN; POLLEN; PROTEINAS; PROTEINE; PROTEINS; PRUNUS AMYGDALUS; SELF POLLINATION; VARIACION GENETICA; VARIATION GENETIQUE
• ISSN: 0013-7626; 1880-358X
• DOI: 10.2503/jjshs.56.300

1. In order to test our hypothesis for the origin and transfer of Sf, a self-fertile gene, as well as for the genetic constitution of several almond cultivars, some preliminary protein analysis on pistils and pollen grains was carried out by isoelectric-focusing and SDS-PAGE in combination with a silver-nitrate staining procedure recently established. Some specific protein bands peculiar to Amygdalus webbii and‘Tuono’were observed. This could indicate the transfer of the Sf gene from the former to the latter. Some specific protein bands on certain cultivars and strains also appeared. These may be related to S2, S3 or S4. We believe that our hypothesis could be proved through further electrophoretic analysis. 2. Artificial pollination was carried out among the 4 cultivars and strains whose genetic constitution is supposed to be S1S3, and the pollen behavior in pistils soon after pollination was investigated by means of fluorescent microscopy. Two remarkable features were observed: namely, bad adhesion of pollen grains stigma surfaces and abnormal tube thinning with poor callose accumulation onto tube walls. This revealed the existence of cross-incompatibility among No. 484, ‘Ferraduel’, ‘Ferralise’and No. 892. It seems that stigmas are very critical in pollination incompatibility of almonds.