Comparison of supercritical fluid chromatography-tandem mass spectrometry and liquid chromatography-tandem mass spectrometry for the stereoselective analysis of chlorfenvinphos and dimethylvinphos in tobacco

• Published in: Journal of separation science Vol. 46; no. 19; p. e2300449
• Main Authors: Yang, Fei, Cui, Haozhe, Wang, Chunqiong, Wang, Ying, Zhu, Wenjing, Deng, Huimin, Liu, Shanshan, Bian, Zhaoyang, Lu, Junli, Tang, Gangling, Ji, Yuan
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.10.2023
• Subjects: Analytical chemistry; Chlorfenvinphos; Chromatography; Chromatography, High Pressure Liquid - methods; Chromatography, Liquid; Chromatography, Supercritical Fluid; Ions; Linearity; Liquid chromatography; Mass spectrometry; Nicotiana - chemistry; Parameter sensitivity; Scientific imaging; Sensitivity; Stereoisomerism; Stereoselectivity; Supercritical fluids; Tandem Mass Spectrometry - methods; Tobacco; Validation studies; chlorfenvinphos; dimethylvinphos; supercritical fluid chromatography-tandem mass spectrometry; stereoselective separation; reversed-phase liquid chromatography-tandem mass spectrometry
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.202300449

This study used reversed-phase liquid chromatography-tandem mass spectrometry and supercritical fluid chromatography-tandem mass spectrometry for determination of the stereoisomers of chlorfenvinphos and dimethylvinphos in tobacco. Tobacco samples were extracted and purified with a modified quick, easy, cheap, effective, rugged, and safe technique using spherical carbon. The performance of both methodologies was comprehensively compared in terms of methods validation parameters (separation efficiency, linearity, selectivity, recovery, repeatability, sensitivity, matrix effect, etc.). Under optimized conditions, the calibration curves of the stereoisomers of chlorfenvinphos and dimethylvinphos in the range of 10-500 ng/mL showed excellent linearity with R ≥  0.997 in both methods. The adequate recoveries of analytes from three different spiked tobaccos were obtained using reversed-phase liquid chromatography-tandem mass spectrometry (86.1-95.7%) as well as supercritical fluid chromatography-tandem mass spectrometry (86.5-94.0%). The relative standard deviations for spiked samples were all below 7.0%. Compared with supercritical fluid chromatography-tandem mass spectrometry, lower matrix effects and LODs can be obtained in reversed-phase liquid chromatography-tandem mass spectrometry.