A perylenediimide based 'on-off' chemosensor for the detection of nucleoside triphosphates: an efficient ensemble for monitoring alkaline phosphatase activity

• Published in: Analytical methods Vol. 11; no. 41; pp. 532 - 5327
• Main Authors: Mittal, Lalit Singh, Sharma, Poonam, Kaur, Navdeep, Singh, Prabhpreet
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 07.11.2019
• Subjects: Adenosine triphosphate; Alkaline phosphatase; Aqueous solutions; ATP; Chemical sensors; Chemoreceptors; CTP; Cytidine triphosphate; Diphosphates; Fluorescence; Guanosine; Guanosine triphosphate; Monitoring; Nucleoside triphosphates; Nucleosides; Optical properties; Phosphatase; Phosphates; Polyphosphates; Quenching; Uridine
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/c9ay01608b

The evaluation of the optical properties of BAB-PDI towards nucleoside polyphosphates (NPPs) in 90% aqueous media (pH 7.2) revealed that upon addition of guanosine triphosphate (GTP), the fluorescence intensity of BAB-PDI was >85% quenched. In the case of adenosine triphosphate (ATP), uridine triphosphate (UTP) and cytidine triphosphate (CTP), only 30-65% quenching of fluorescence intensity was observed. Other NPPs such as nucleoside diphosphates and monophosphates, respectively, caused 20-30% and <5% quenching of fluorescence intensity of BAB-PDI due to fewer negative charges on di- and mono-phosphates. The minimum limit of detection of GTP reached 19.3 nM. The application of BAB-PDI in monitoring enzyme (alkaline phosphatase) activity in aqueous solution using a BAB-PDI + GTP ensemble has also been demonstrated. Cationic perylenediimide ( BAB-PDI ) showed fluorescence sensing toward nucleoside polyphosphates (GTP, LOD = 19.3 nM) in an aqueous medium. A BAB-PDI + GTP ensemble could be used to monitor the activity of alkaline phosphatase enzyme.