Assessment of diversity, distribution and antibacterial activity of endophytic fungi isolated from a medicinal plant Adenocalymma alliaceum Miers

• Published in: Symbiosis (Philadelphia, Pa.) Vol. 55; no. 1; pp. 39 - 46
• Main Authors: Kharwar, Ravindra N., Verma, Satish K., Mishra, Ashish, Gond, Surendra K., Sharma, Vijay K., Afreen, Talat, Kumar, Anuj
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2011; Balaban
• Subjects: Animal, plant and microbial ecology; Biological and medical sciences; Biomedical and Life Sciences; Developmental Biology; Ecology; Evolutionary Biology; Fundamental and applied biological sciences. Psychology; Life Sciences; Microbiology; Plant Sciences; Endophytic fungi; Antibacterial; Colonization frequency; Diversity; Symbiosis; Adenocalymma alliaceum; Interspecific association; Medicinal plant; Fungi; Endophyte; Antibacterial agent; Colonization; Interspecific relation
• ISSN: 0334-5114; 1878-7665
• DOI: 10.1007/s13199-011-0142-2

A study was conducted for isolation, identification and antibacterial potential of fungal endophytes of Adenocalymma alliaceum Miers., (Bignoniaceae), a medicinal shrub vine plant which has long history for its usages in curing various disorders. A total of 149 isolates of endophytic fungi representing 17 fungal taxa were obtained from 270 segments (90 from each stem, leaf and petiole) of this plant. Hyphomycetes (77.85%) were the most prevalent, followed by Ascomycetes (8.05%) and Coelomycetes (4.03%) respectively. A considerable amount of fungal isolates was kept under (10.07%) Mycelia-Sterilia (MS). Leaf harboured maximum colonization of endophytic fungi (72.22%) which was greater than stem (67.78%) and petiole (25.54%). The Jc similarity index was maximum (0.619) between stem vs leaf followed by leaf vs petiole (0.571) and stem vs petiole (0.428). The dominant endophytic fungi were Alternaria alternata, Aspergillus niger, Stenella agalis, Fusarium oxysporum, Curvularia lunata and Fusarium roseum. Among twelve endophytic fungi tested for antibacterial activity, crude extracts of nine endophytic fungi (75%), showed antibacterial potential against one or more clinical human pathogens. Alternaria alternata, Curvularia lunata, Penicillium sp. and Chaetomium globosum exhibited significant antibacterial activity against 4 of 5 tested pathogens, showing broad spectrum activity. This investigation explains the value of sampling from different tissues of a host plant for the greater species diversity, and additionally, the antibacterial screening of some endophytic fungi from this specific medicinal plant may represent a unique source for many of the useful antibacterial compounds.