Enhanced biodesulfurization by magnetic immobilized Rhodococcus erythropolis LSSE8-1-vgb assembled with nano-γ-Al2O3

• Published in: World journal of microbiology & biotechnology Vol. 27; no. 2; pp. 299 - 305
• Main Authors: Zhang, Ting, Li, Wang-Liang, Chen, Xiao-Xin, Tang, Huang, Li, Qiang, Xing, Jian-Min, Liu, Hui-Zhou
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.02.2011; Springer
• Subjects: Applied Microbiology; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Environmental Engineering/Biotechnology; Fundamental and applied biological sciences. Psychology; General aspects; Immobilization techniques; Life Sciences; Methods. Procedures. Technologies; Microbiology; Original Paper; Biodesulfurization; Nano-γ-Al; Magnetic immobilization; In-situ integrated desulfurization; O; Desulfurization; Magnetic; In situ; Immobilization; Bacteria; Rhodococcus erythropolis; Actinomycetes
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-010-0459-7

Biodesulfurization activity can be enhanced by assembling nano-γ-Al 2 O 3 particles on the magnetic immobilized Rhodococcus erythropolis LSSE8-1-vgb. The cells can be collected and reused conveniently by an external magnetic field. Firstly, cells were magnetic immobilized by coating with Fe 3 O 4 nano-particles. The optimal ratio of cells to magnetic Fe 3 O 4 nano-particles was determined to be 50:1 (g/g). Then nano-γ-Al 2 O 3 adsorbents were assembled onto the cells to enhance the desulfurization activity. The nano-γ-Al 2 O 3 adsorbent had the largest pore volume as well as specific surface area, and the strongest electrostatics interaction with microbial cell, and cells assembled with this nano-adsorbent performed the highest desulfurization activity. The activity of magnetic immobilized cells assembled with adsorbents was tested in desulfurization of model oil. The desulfurization rate was raised by nearly 20% when the amount ratio of magnetic particles to adsorbents was 1:5 (g/g). These cells can be reused. The activity decreased less than 10% through out three desulfurization-activation-reuse recycles.