Evaluation of Sempervivum tectorum L. Flavonoids by LC and LC–MS

• Published in: Chromatographia Vol. 68; no. Suppl 1; pp. 107 - 111
• Main Authors: Alberti, Agnes, Blazics, Balazs, Kery, Agnes
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Wiesbaden Vieweg Verlag 01.10.2008; Springer
• Subjects: Analytical Chemistry; Biological and medical sciences; Chemistry; Chemistry and Materials Science; Chromatography; Full Short Communication; General pharmacology; Laboratory Medicine; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Pharmacy; Proteomics; Hungary; Europe; Kaempferol glycosides; Flavonoids; Column liquid chromatography–mass spectrometry; L; Tandem mass spectrometry; Chemical analysis; Crassulaceae; HPLC chromatography; Flavonoid; Disaccharide; Electrospray; Medicinal plant; Sempervivum tectorum L; Polyphenol; Folk medicine; Dicotyledones; Column liquid chromatography-mass spectrometry; Angiospermae; Glycoside; Quantitative analysis; Elution; Gradient; Aldose; Kaempferol; Coupled method; Pharmacognosy; Plant leaf; Reversed phase chromatography; Ultraviolet detector; Plant origin; Flavone derivatives; Spermatophyta
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1365/s10337-008-0750-z

Flavonoid profile of houseleek has been revealed and the content of its phenolic compounds has been determined. Polar extracts (80% ( v/v ) methanol) of lyophylized Sempervivum leaves have been studied. LC separation of fractions was performed on a Supelcosil LC18 (250 × 4.6 mm; 5 μm) column. For linear gradient elution the following solvent mixture was applied: methanol −2.5% ( v/v ) acetic acid. The presence of numerous flavonoid compounds was detected by their retention times and UV spectra. Flavonoid compounds were evaluated with LC–ESI–MS–MS, in the negative ion mode, kaempferol was detected as main flavonoid aglycone, occuring solely in glycosidic form.