Urokinase-type plasminogen activator and plasminogen activator inhibitor antigen in tissue extracts of paranasal sinus mucous membranes affected by chronic sinusitis and antrochoanal polyps

• Published in: European archives of oto-rhino-laryngology Vol. 256; no. 5; pp. 237 - 241
• Main Authors: SUNAGAWA, M, KINJOH, K, NAKAMURA, M, KOSUGI, T
• Format: Journal Article
• Language: English
• Published: Berlin Springer 1999
• Subjects: Antigens - analysis; Biological and medical sciences; Buffers; Chronic Disease; Culture Techniques; Enzyme-Linked Immunosorbent Assay; Humans; Hydrogen-Ion Concentration; Maxillary Sinus - chemistry; Maxillary Sinus - enzymology; Medical sciences; Mucous Membrane - chemistry; Nasal Mucosa - chemistry; Nasal Mucosa - metabolism; Nasal Polyps - chemistry; Nasal Polyps - enzymology; Non tumoral diseases; Otorhinolaryngology. Stomatology; Plasminogen Activator Inhibitor 1 - analysis; Sinusitis - enzymology; Sinusitis - immunology; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology; Urokinase-Type Plasminogen Activator - analysis; Urokinase-Type Plasminogen Activator - metabolism; Human; Nose disease; u-Plasminogen activator; Serine endopeptidases; Enzyme; Fibrinolysis; Polyposis; Sinusitis; Peptidases; Plasminogen activator inhibitor 1; Tissue; Chronic; Nose; Hydrolases; ENT disease; Paranasal sinus disease; Benign neoplasm; Quantitative analysis
• ISSN: 0937-4477; 1434-4726
• DOI: 10.1007/s004050050149

We examined the effect of pH on the extraction of urokinase-type plasminogen activator (u-PA) and plasminogen activator inhibitor-1 (PAI-1) from paranasal sinus mucous membrane associated with chronic sinusitis and antrochoanal polyps. The specific activity of u-PA extracted with buffer at pH 7.4 was stronger than that extracted with buffer at pH 4.2. The antigen level of u-PA extracted with the acidic buffer was significantly higher than that extracted with the neutral buffer. In contrast, the difference in antigen levels of PAI-1 extracted with the acidic buffer and neutral buffer was not significant. Based on these results, we inferred that the u-PA-PAI-1 complex was extracted by the acidic buffer and the activity of u-PA was therefore decreased.