Advances and prospects of transcription‐factor‐based biosensors in high‐throughput screening for cell factories construction

Non/semi‐rational engineering strategies can improve the performance of microbial cell factories under the situations of indistinct metabolic regulations and interactions. However, generating positive variants through these strategies is remarkably low. Using traditional screening methods to evaluat...

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Bibliographic Details
Published inFood bioengineering Vol. 1; no. 2; pp. 135 - 147
Main Authors Li, Changfan, Wang, Chang, Zhu, Jiang, Xue, Feng, Sun, Xiaoman, Gu, Yang
Format Journal Article
LanguageEnglish
Published Shanghai John Wiley & Sons, Inc 01.06.2022
Wiley
Subjects
Biosensors
Construction engineering
E coli
Engineering
Evolution & development
Factories
Fatty acids
high‐throughput screening
Industrial engineering
Manufacturing engineering
Mass spectrometry
Mass spectroscopy
Metabolic engineering
Metabolism
microbial cell factories
Microorganisms
Performance enhancement
Performance evaluation
Screening
Sensors
transcription factor
Yeast
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Summary:Non/semi‐rational engineering strategies can improve the performance of microbial cell factories under the situations of indistinct metabolic regulations and interactions. However, generating positive variants through these strategies is remarkably low. Using traditional screening methods to evaluate the performance of mutants, such as mass spectrometry and chromatography, is low‐throughput, slow‐detection, and labor‐intensive. Specifically, the efficiency of high‐throughput screening (HTS) for strain screening could reach even more 103−106 strains/day, bridging the gap between non/semi‐rational engineering strategies and microbial cell factories construction. This review highlights transcription factor (TFs)‐based biosensors in the advances in developing synthetic biosensors for HTS. We hope that this review will help take full advantage of valuable TFs to guide the HTS technology and promote its development. Transcription factors (TFs) have been successfully used for high‐throughput screening (HTS) of high‐yielding strains, avoiding tedious and time‐consuming detection. This review highlights the advances in developing synthetic biosensors for HTS, focusing on TFs‐based biosensors.
ISSN:2770-2081
2770-2081
DOI:10.1002/fbe2.12019