Heme O is present in Paracoccus denitrificans cells and accumulates under anoxic growth

• Published in: FEMS microbiology letters Vol. 190; no. 1; pp. 63 - 66
• Main Authors: Kaplan, Petr, Erlebachová, Petra, Kucčera, Igor, Čáslavský, Josef
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2000; Blackwell; Oxford University Press
• Subjects: Aeration; Anaerobiosis; Bacteriology; Biological and medical sciences; Cell culture; Chromatography, High Pressure Liquid - methods; E coli; Fundamental and applied biological sciences. Psychology; Heme; Heme - metabolism; Heme group; High performance liquid chromatography; Ions; Liquid chromatography; MALDI‐TOF mass spectrometry; Mass spectrometry; Mass spectroscopy; Metabolism. Enzymes; Microbiology; Paracoccus denitrificans; Paracoccus denitrificans - growth & development; Paracoccus denitrificans - metabolism; Reverse‐phase HPLC; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; MALDI-TOF mass spectrometry; Heme group; Reverse-phase HPLC; Anaerobiosis; Aerobiosis; Heme; Halomonas denitrificans; Bacteria; Environmental factor; Biosynthesis; Metabolism; Halomonadaceae
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2000.tb09263.x

Abstract In spite of the published claims to the contrary, Paracoccus denitrificans was shown to contain a heme derivative, virtually indistinguishable from the Escherichia coli heme O on the basis of the reverse-phase high-performance liquid chromatography and MALDI-TOF mass spectrometry analyses. Aeration of the anoxically grown culture resulted in the disappearance of a significant portion of this compound with concomitant build-up of heme A.