Tacaribe Virus Infection May Induce Inhibition of the Activity of the Host Cell Ca2+ and Na+/K+ Pumps

• Published in: Journal of general virology Vol. 69; no. 4; pp. 951 - 954
• Main Authors: Rey, Osvaldo, Rossi, Juan P. F. C, Lopez, Ricardo, Iapalucci-Espinoza, Silvia J, Franze-Fernandez, Maria T
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.04.1988; Society for General Microbiology
• Subjects: Animals; Arenaviridae - physiology; Biological and medical sciences; Calcium-Transporting ATPases - antagonists & inhibitors; Defective Viruses - physiology; Fundamental and applied biological sciences. Psychology; Microbiology; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors; Vero Cells; Virology; Virus Replication; Cell culture; Tacaribe virus; Calcium; Cell permeability; Host virus relation; Virus; Membrane pump; Sodium; Inhibition; Defectivity; Potassium; Arenaviridae; Self interference
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-69-4-951

Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Junín 956, 1113 Buenos Aires, Argentina Infection of Vero cells with Tacaribe virus stocks containing a high ratio of standard (plaque-forming) viruses to defective interfering particles (DIP) induced inhibition of the host cell Ca 2+ ATPase (Ca 2+ pump) and the ouabain-sensitive Na + /K + ATPase (Na + /K + pump). The Mg 2+ ATPase which is not involved in cation transport was not affected. The presence of DIP in the inocula protected the cells from alteration of the transport-associated ATPases induced by standard viruses. Keywords: Tacaribe virus, defective interfering particles, cation pumps Present address: Centro de Virología Animal, Serrano 665, 1414 Buenos Aires, Argentina. Received 6 October 1987; accepted 11 January 1988.