Immunohistochemical characterization of renal amyloidosis

• Published in: American journal of clinical pathology Vol. 87; no. 6; pp. 756 - 761
• Main Authors: NOEL, L.-H, DROZ, D, GANEVAL, D
• Format: Journal Article
• Language: English
• Published: Chicago, IL American Society of Clinical Pathologists 01.06.1987
• Subjects: Amyloidosis - classification; Amyloidosis - diagnosis; Amyloidosis - metabolism; Biological and medical sciences; Biopsy; Fluorescent Antibody Technique; Histocytochemistry; Humans; Kidney - metabolism; Kidney - pathology; Kidney Diseases - classification; Kidney Diseases - diagnosis; Kidney Diseases - metabolism; Kidneys; Medical sciences; Nephrology. Urinary tract diseases; Staining and Labeling - methods; Urinary system involvement in other diseases. Miscellaneous; Human; Immunohistochemistry; Urinary system disease; Biopsy; Systemic disease; Amyloidosis; Kidney
• ISSN: 0002-9173; 1943-7722
• DOI: 10.1093/ajcp/87.6.756

Forty-five renal biopsies with amyloidosis were studied by light microscopy with Congo red staining and action of potassium permanganate and by immunofluorescence with antihuman tissue A component antiserum antilight and heavy chains of immunoglobulins antisera. The patients were classified on the basis of concordance between immunohistochemical characterization by immunofluorescence and the results of Congo red staining after potassium permanganate treatment. Thus, 37 of 45 cases (82%) were classified by immunohistochemical characterization (15 with AL amyloidosis and 22 with AA amyloidosis) when the amyloid type could be hypothetized in only 31 of these cases (66%) on the basis of clinical criteria. This study suggests that the association of these two technics is more reliable than clinical data alone in distinguishing between AA and AL amyloidosis.