Development of chitosan/poly-γ-glutamic acid/pluronic/curcumin nanoparticles in chitosan dressings for wound regeneration

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Vol. 105; no. 1; pp. 81 - 90
• Main Authors: Lin, Yu-Hsin, Lin, Jui-Hsiang, Hong, Ya-Shiuan
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.01.2017
• Subjects: Acids; Bacterial diseases; Biocompatibility; Biomedical materials; Cells, Cultured; Chitosan; Chitosan - chemistry; Chitosan - pharmacology; Computer simulation; Curcumin; Curcumin - chemistry; Curcumin - pharmacology; Fibroblasts - metabolism; Fibroblasts - pathology; Gelation; Glutamic acid; Humans; Hydrophobicity; In vivo methods and tests; Inflammation; Materials research; Materials science; Materials Testing; Medical dressings; Membranes; Nanoparticles; Nanoparticles - chemistry; Poloxamer - chemistry; Poloxamer - pharmacology; Polyglutamic Acid - chemistry; Polyglutamic Acid - pharmacology; Polymers; Regeneration; Skin; Wound healing; Wound Healing - drug effects; Zeta potential; chitosan; nanoparticles; wound regeneration; pluronic; curcumin
• ISSN: 1552-4973; 1552-4981
• DOI: 10.1002/jbm.b.33394

The hydrophobic polyphenol curcumin has anti-inflammatory, antimicrobial, and wound-healing properties that warrant its pharmacological consideration. We report a curcumin nanoparticle with a tripolymeric composite that can be used as a delivery device for wound healing. The present composite nanoparticles were prepared with three biocompatible polymers of chitosan, poly-γ-glutamic acid, and pluronic using a simple ionic gelation technology. Pluronic was used to enhance the solubility of curcumin in chitosan/poly-γ-glutamic acid nanoparticles, leading to the incorporation of chitosan/poly-γ-glutamic acid/pluronic/curcumin nanoparticles into chitosan membranes, and reduced inflammation and bacterial infection during wound regeneration. Nanoparticles were of 193.1 ± 8.9 nm and had a zeta potential of 20.6 ± 2.4 mV. Moreover, in vitro analyses indicated controlled curcumin release in a simulated skin tissue model. Subsequent in vivo studies show that chitosan wound dressing containing chitosan/poly-γ-glutamic acid/pluronic/curcumin nanoparticles promoted neocollagen regeneration and tissue reconstruction. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 81-90, 2017.