Localization of ATPase activity in striated muscle and probable sources of artifact

ABSTRACT An attempt has been made to localize sites of ATPase activity in stretched fibrils from glycerinated rabbit muscle by precipitating the liberated phosphate with lead ions. As a control, the distribution of lead phosphate grains precipitated by adding phosphate solutions to fibrils incubated...

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Bibliographic Details
Published inJournal of cell science Vol. 2; no. 1; pp. 113 - 118
Main Authors Gillis, J M, Page, S G
Format Journal Article
LanguageEnglish
Published England 01.03.1967
Subjects
Adenosine Triphosphatases - metabolism
Adenosine Triphosphate
Animals
Chemical Precipitation
Glycerol
Histocytochemistry
Lead
Microscopy, Electron
Muscle Proteins
Muscles - enzymology
Phosphates
Rabbits
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Summary:ABSTRACT An attempt has been made to localize sites of ATPase activity in stretched fibrils from glycerinated rabbit muscle by precipitating the liberated phosphate with lead ions. As a control, the distribution of lead phosphate grains precipitated by adding phosphate solutions to fibrils incubated with lead (without ATP) has been studied. In these conditions, the grains are not uniformly distributed along the fibrils, but show many of the features of the pattern of distribution that is found after the fibres have been incubated in ATP and lead. It is suggested that differential lead binding by the protein filaments, or movement of the grains, may be responsible for this non-uniform distribution, and that consequently no conclusions concerning sites of ATPase activity within the sarcomere can be drawn from the results of methods using lead precipitation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0021-9533
1477-9137
DOI:10.1242/jcs.2.1.113