Metal-organic framework templated synthesis of Co3O4 nanoparticles for direct glucose and H2O2 detection

• Published in: Analyst (London) Vol. 137; no. 24; pp. 5803 - 5808
• Main Authors: Hou, Chuantao, Xu, Qin, Yin, Lina, Hu, Xiaoya
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 21.12.2012
• Subjects: Analytical chemistry; Biological and medical sciences; Biosensors; Biotechnology; Chemistry; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; Methods. Procedures. Technologies; Spectrometric and optical methods; Various methods and equipments; Performance evaluation; Fourier transform spectroscopy; Nanoparticle; Selectivity; Glucose; Metal; Blood; Electrocatalysis; Detection limit; Basic medium; Serum; Oxidation; Human; Scanning electron microscopy; Enzyme; Sample; Use; Carbon electrode; Concentration; Chemical sensor; X ray diffraction; Infrared spectrometry; Enzymatic method; Sensitivity; Transmission electron microscopy; Response time; Biosensor; Poison; Detection; Application
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/c2an35954e

Co(3)O(4) nanoparticles (NPs) with an average diameter of about 20 nm were synthesized by using MOFs as a template. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed to characterize the as-prepared Co(3)O(4) NPs. Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) were used to confirm the structure of the Co(3)O(4) NPs. Then the Co(3)O(4) NPs were modified on a glassy carbon electrode (GCE) to obtain a non-enzymatic glucose and H(2)O(2) sensor. The NPs show electrocatalytic activity toward oxidation of glucose and H(2)O(2) in alkaline medium. For glucose detection, the developed sensor shows a short response time (less than 6 s), a high sensitivity of 520.7 μA mM(-1) cm(-2), a detection limit of 0.13 μM (S/N = 3), and good selectivity. The high concentration of NaCl does not poison the electrode. Its application for the detection of glucose in a human blood serum sample shows good agreement with the results obtained from the hospital. Furthermore, the proposed sensor was used for the detection of H(2)O(2). The results indicate that the detection limit and sensitivity for H(2)O(2) are 0.81 μM and 107.4 μA mM(-1) cm(-2), respectively. Determination of H(2)O(2) concentration in a disinfectant sample by the proposed biosensor also showed satisfactory result. The high sensitivity and low detection limit can be attributed to the excellent electrocatalytic performance of the as-prepared Co(3)O(4) NPs. These results demonstrate that the as-prepared Co(3)O(4) NPs have great potential applications in the development of sensors for enzyme-free detection of glucose and H(2)O(2).