Responses of catecholestrogen metabolism to acute graded exercise in normal menstruating women before and after training

• Published in: The journal of clinical endocrinology and metabolism Vol. 82; no. 10; pp. 3342 - 3348
• Main Authors: DE CREE, C, BALL, P, SEIDLITZ, B, VAN KRANENBURG, G, GEURTEN, P, KEIZER, H. A
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01.10.1997
• Subjects: Adolescent; Adult; Biological and medical sciences; Epinephrine - blood; Estrogens - blood; Estrogens, Catechol - blood; Exercise; Female; Follicular Phase - blood; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; Humans; Luteal Phase - blood; Mammalian female genital system; Menstruation - blood; Norepinephrine - blood; Physical Education and Training; Reference Values; Vertebrates: reproduction; Physical exercise; Human; Menstrual cycle; Estrogen; Biosynthesis; Metabolism; Maximal physical exercise; Female; Biological effect; Hormonal investigation; Sex steroid hormone; Catechol estrogen; Comparative study
• ISSN: 0021-972X; 1945-7197
• DOI: 10.1210/jc.82.10.3342

It has been hypothesized that exercise-related hypo-estrogenemia occurs as a consequence of increased competition of catecholestrogens (CE) for catechol-O-methyltransferase (COMT). This may result in higher norepinephrine (NE) concentrations, which could interfere with normal gonadotropin pulsatility. The present study investigates the effects of training on CE responses to acute exercise stress. Nine untrained eumenorrheic women (mean percentage of body fat +/-SD: 24.8 +/- 3.1%) volunteered for an intensive 5-day training program. Resting, submaximal, and maximal (tmax) exercise plasma CE, estrogen, and catecholamine responses were determined pre- and post training in both the follicular (FPh) and luteal phase (LPh). Acute exercise stress increased total primary estrogens (E) but had little effect on total 2-hydroxyestrogens (2-OHE) and 2-hydroxyestrogen-monomethylethers (2-MeOE) (= O-methylated CE after competition for catechol-O-methyltransferase). This pattern was not significantly changed by training. However, posttraining LPh mean (+/-SE) plasma E, 2-OHE, and 2-MeOE concentrations were significantly lower (P < 0.05) at each exercise intensity (for 2-OHE: 332 +/- 47 vs. 422 +/- 57 pg/mL at tmax; for 2-MeOE: 317 +/- 26 vs. 354 +/- 34 pg/mL at tmax). Training produced opposite effects on 2-OHE:E ratios (an estimation of CE formation) during acute exercise in the FPh (reduction) and LPh (increase). The 2-MeOE:2-OHE ratio (an estimation of CE activity) showed significantly higher values at tmax in both menstrual phases after training (FPh: +11%; LPh: +23%; P < 0.05). After training, NE values were significantly higher (P < 0.05). The major findings of this study were that: training lowers absolute concentrations of plasma estrogens and CE; the acute exercise challenge altered plasma estrogens but had little effect on CE; estimation of the formation and activity of CE suggests that formation and O-methylation of CE proportionately increases. These findings may be of importance for NE-mediated effects on gonadotropin release.