Atmnd1-Δ1 is sensitive to gamma-irradiation and defective in meiotic DNA repair

• Published in: DNA repair Vol. 5; no. 4; pp. 455 - 464
• Main Authors: Domenichini, Séverine, Raynaud, Cécile, Ni, Di-An, Henry, Yves, Bergounioux, Catherine
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.04.2006; Elsevier
• Subjects: Bacteriology; Biological and medical sciences; DSB repair; Fundamental and applied biological sciences. Psychology; Growth, nutrition, cell differenciation; Meiosis; Microbiology; Molecular and cellular biology; Molecular genetics; Mutagenesis. Repair; Rad51; Rad51; DSB repair; Meiosis; DSB repair Meiosis Rad51; Gamma irradiation; Repair; DNA
• ISSN: 1568-7864; 1568-7856
• DOI: 10.1016/j.dnarep.2005.12.007

The efficient repair of double-strand breaks (DSBs) in genomic DNA is crucial for the survival of all organisms. Mnd1 is suggested to promote the strand invasion step during meiotic recombination. We used a forward genetics approach, through the search for mutants, to characterize the Arabidopsis homologue of Mnd1. Atmnd1 null mutants exhibit normal vegetative and flower development. In contrast, during prophase I, chromosomes become fragmented resulting in random distribution of the fragments between polyads. Male and female meiosis are defective and strong accumulation of AtRAD51 was observed in atmnd1-Δ1 nuclei. These results suggest that similarly to its yeast and animal homologues, AtMnd1 plays a role in DSB repair during meiosis. In addition, like its human homologue AtMnd1 is expressed in somatic cells. AtMnd1 expression is strongly induced by γ-rays and null mutants show increased sensibility to γ-rays. Taken together, these results suggest that AtMnd1 is also required for DSB repair in somatic cells.