Correlation of DNA content and histology in prognosis of astrocytomas

Thirty-two cases of astrocytoma were analyzed for DNA content and cell-cycle proliferation features by flow cytometry using paraffin-embedded tissue. The findings were correlated with histologic grading and survival. Abnormal DNA (aneuploidy or elevated G2-M fraction greater than or equal to 7%) was...

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Bibliographic Details
Published inAmerican journal of clinical pathology Vol. 90; no. 3; pp. 289 - 292
Main Authors COONS, S. W, DAVIS, J. R, WAY, D. L
Format Journal Article
LanguageEnglish
Published Chicago, IL American Society of Clinical Pathologists 01.09.1988
Subjects
Aneuploidy
Astrocytoma - analysis
Astrocytoma - pathology
Biological and medical sciences
Diploidy
DNA - analysis
Flow Cytometry
Glioblastoma - analysis
Glioblastoma - pathology
Humans
Medical sciences
Neurology
Prognosis
Tumors of the nervous system. Phacomatoses
Human
Flow cytometry
Intracranial
Ploidy
Nervous system diseases
Prognosis
Glioblastoma multiforme
Survival
Pathology
DNA
Cell cycle
Tumor
Nucleic acid
Astrocytoma
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Summary:Thirty-two cases of astrocytoma were analyzed for DNA content and cell-cycle proliferation features by flow cytometry using paraffin-embedded tissue. The findings were correlated with histologic grading and survival. Abnormal DNA (aneuploidy or elevated G2-M fraction greater than or equal to 7%) was present in 18 cases (56%). Glioblastoma multiforme (GBM) had 11 of 16 (69%), anaplastic astrocytomas (ANA) 7 of 11 (64%), and low-grade (LG) neoplasms 0 of 5 cases with abnormal DNA content. Short-term survival (less than or equal to 26 months) occurred in all 16 patients with GBM (100%), 7 of 11 patients with ANA (64%), and 1 of 5 patients with LG neoplasms (20%). Seventeen of 18 patients (94%) with abnormal DNA content were short-term survivors (P less than 0.0002). Abnormal DNA content was found in 17 of 24 short-term survivors (71%), whereas histologic grading identified 16 of 24 such cases (67%). A combination of grading and abnormal DNA content identified 22 of 24 (92%) of the poor survival cases. DNA content was most useful in the anaplastic group. Six of seven cases (86%) with abnormal DNA content had short survival (P less than 0.055), and three of four (75%) with normal DNA content had long survival. DNA analysis combined with histologic grading improves prognosis designation.
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ISSN:0002-9173
1943-7722
DOI:10.1093/ajcp/90.3.289