CD44 splice variants in draining lymph nodes precede allograft rejection of endocrine cells

• Published in: Journal of molecular medicine (Berlin, Germany) Vol. 77; no. 1; pp. 67 - 69
• Main Authors: MILDE, K. F, HERRLICH, P, PONTA, H
• Format: Conference Proceeding Journal Article
• Language: English
• Published: Berlin Springer 1999
• Subjects: Alternative Splicing; Animals; Biological and medical sciences; Exons; Graft Rejection; Hyaluronan Receptors - genetics; Hyaluronan Receptors - immunology; Islets of Langerhans Transplantation - immunology; Lymph Nodes - immunology; Lymphocytes - immunology; Medical sciences; Miscellaneous; Molecular Sequence Data; Rats; Rats, Inbred Lew; Rats, Inbred WF; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - analysis; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Transplantation, Homologous; Endocrinopathy; Graft(material); Lymph node; Rat; Transcription; Diabetes mellitus; Genetic variant; Rodentia; Langerhans islet; Homograft; Biological marker; Rejection; Vertebrata; Mammalia; Endocrine cell; Treatment; Surgery; Animal; Graft; Complication; Molecular biology; Endocrine pancreas
• ISSN: 0946-2716; 1432-1440
• DOI: 10.1007/s001090050303

Upon allogeneic transplantation (Tx) of pancreatic islets under the kidney capsule of diabetic rats, cells from draining lymph nodes and, to a minor degree, bone marrow transiently upregulate CD44 splice variants as detected by RT-PCR using CD44 variant exon specific primers. Maximal expression was on day 5 post Tx in lymph nodes and thus precedes islet rejection sufficiently (in this model by 5 days) to still permit establishing rescue by immunosuppressive therapy. CD44 variant exon sequence could therefore serve as early markers of allograft rejection.