Effect of tibolone on turnover of cholesterol to bile acids in ovariectomized rats

• Published in: Menopause (New York, N.Y.) Vol. 12; no. 5; p. 609
• Main Authors: Czerny, Bogusław, Teister, Maria, Juzyszyn, Zygmunt, Mysliwiec, Zofia, Pawlik, Andrzej
• Format: Journal Article
• Language: English
• Published: United States 01.09.2005
• Subjects: Animals; Bile Acids and Salts - biosynthesis; Carbon Radioisotopes; Cholesterol - metabolism; Estrogen Receptor Modulators - pharmacology; Female; Norpregnenes - pharmacology; Ovariectomy; Rats; Rats, Wistar
• ISSN: 1072-3714
• DOI: 10.1097/01.gme.0000178449.52248.39

Tibolone is used in breast cancer therapy in women. The most important aspect of this therapy concerns its influence on bone and lipid metabolism. Several studies have reported the effect of tibolone on plasma lipids. Approximately 40% of total cholesterol removal occurs by its conversion to bile acids, a process that takes place in the liver. The aim of the study was to evaluate the influence of tibolone on the conversion of cholesterol to bile acids in estrogen-deficient rats. Thirty female Wistar rats were divided into three groups: sham-operated controls, ovariectomized controls, and ovariectomized rats treated with tibolone. After 42 days of drug administration, bile was collected under anesthesia after administration of radioactive 4-C cholesterol. Bile was assayed for total C radioactivity; C bile acids were determined after thin-layer chromatography separation by using the isotopic technique. The ovariectomy decreased the concentrations of C-radioactive bile, taurocholic acid, and cholic acid. The administration of tibolone significantly increased the excretion of C-radioactive bile and C-radioactive bile acids as compared with ovariectomized controls. Moreover, the therapy with tibolone significantly increased the concentrations of C taurocholic acid, C glycocholic acid, cholic acid, and taurochenodeoxycholic plus taurodeoxycholic acids. The results of the present study suggest that tibolone may increase the concentrations of cholesterol and trihydroxy bile acids in bile. These changes may be associated with an increased risk of gallstone generation.