Implanting of Single Zinc Sites into 2D Metal–Organic Framework Nanozymes for Boosted Antibiofilm Therapy

• Published in: Advanced functional materials Vol. 33; no. 10
• Main Authors: Wang, Xiaoyu, Hu, Wenchao, Xia, Xing‐Hua, Wang, Chen
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc 01.03.2023
• Subjects: antibiofilm therapies; Density functional theory; Hydrogen peroxide; Hydroxyl radicals; In vivo methods and tests; Materials science; Metal-organic frameworks; nanozymes; Peroxidase; Porphyrins; single‐site catalysis; Surfactants; Two dimensional materials; Zinc
• ISSN: 1616-301X; 1616-3028
• DOI: 10.1002/adfm.202212798

Design of nanozymes with catalytic active sites at atomic‐scale not only improves the atomic utilization, but also provides a well‐defined coordination structure for nanozymes’ catalytic mechanism research. Herein, a surfactant‐assisted method is reported for preparing 2D metal–organic frameworks based single zinc sites nanozyme (SZN‐MOFs) through assembling preformed Zn single‐atom coordinated porphyrin precursors into ultrathin MOF nanosheets. The Zn atom loading weight ratio in SZN‐MOFs is up to 4.6 wt.%. The SZN‐MOFs exhibit extraordinary peroxidase‐like activity, which can effectively catalyze H2O2 into hydroxyl radicals. The catalytical mechanism is elucidated and the origin of the high peroxidase‐like activity of SZN‐MOFs is rationalized using density functional theory calculations. Finally, it is demonstrated that the SZN‐MOFs present great promises for in vitro and in vivo antibiofilm activity under a low concentration of H2O2. This study not only develops a surfactant‐assisted method for fabricating MOF‐based single sites nanozyme, but also manifests the applications in the field of antibiofilm therapy. A surfactant‐assisted coordination strategy is used to synthesize 2D metal–organic frameworks based single zinc sites nanozyme (SZN‐MOFs) with a high zinc loading of 4.6 wt.%, by assembly of pre‐formed single zinc atom coordinated porphyrin precursors into ultrathin MOF nanosheets. The SZN‐MOFs exhibit extraordinary peroxidase‐like activity and present great promises for in vitro and in vivo antibiofilm activity.