Screen‐printed Nickel‐Cerium Hydroxide Sensor for Acetaminophen Determination in Body Fluids

• Published in: ChemElectroChem Vol. 8; no. 13; pp. 2505 - 2511
• Main Authors: Azeredo, Nathália F. B., Gonçalves, Josué M., Lima, Irlan S., Araki, Koiti, Wang, Joseph, Angnes, Lúcio
• Format: Journal Article
• Language: English
• Published: Weinheim John Wiley & Sons, Inc 01.07.2021
• Subjects: acetaminophen; alpha-nickel-cerium hydroxide; Analgesics; Body fluids; Cerium; Diameters; Electrochemical analysis; Graphite; nanocatalysts; Nanoparticles; Nickel; Reagents; Saliva; screen-printed electrodes; Selectivity; Sweat; Uric acid
• ISSN: 2196-0216; 2196-0216
• DOI: 10.1002/celc.202100417

The possibility of fast, reliable, and sensitive (μmol L−1) quantification of acetaminophen (AAP) directly in saliva and sweat was demonstrated in the pH range of 4 to 7 using a new user‐friendly and cost‐effective graphite screen‐printed electrode (SPE) modified with 2–3 nm diameter alpha‐nickel‐cerium hydroxide nanoparticles. The nanoparticles (NPs) were fully characterized by transmission electron microscopy (TEM, HRTEM and STEM), XRD, and the electrochemical conditions for AAP analysis optimized, pushing down the limit of detection with good selectivity. The possibility of real‐time measurements of AAP was demonstrated by carrying out experiments directly in biofluids, without significant interference from species such as lactate, glucose, ascorbic and uric acid usually present in this kind of samples. This is the first report combining the catalytic properties and easy incorporation of alpha‐nickel‐cerium hydroxide nanoparticles into graphite ink to prepare an SPE for AAP electrochemical detection in secreting body fluids. The α‐Ni0.9Ce0.1(OH)2 SPE features good accuracy and selectivity, fast response, good accuracy and selectivity, as well as low reagent consumption for determination of AAP in real saliva and sweat samples. Fast, reliable, and sensitive quantification of acetaminophen (AAP) in saliva and sweat is demonstrated using a convenient and cost‐effective graphite screen‐printed electrode modified with alpha‐nickel‐cerium hydroxide nanoparticles.