Solid-phase synthesis of 2'-deoxy-2'-fluoro- beta-D-oligoarabinonucleotides (2'F-ANA) and their phosphorothioate derivatives

This unit describes the chemical synthesis of 2'-deoxy-2'-fluoro-b-D-oligoarabinonucleotides (2'F-ANA), both with phosphodiester and phosphorothioate linkages. The protocols described herein include araF phosphoramidite preparation, assembly on DNA synthesizers, and final deprotection...

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Bibliographic Details
Published inCurrent protocols in nucleic acid chemistry Vol. Chapter 4; p. Unit 4.15
Main Authors Viazovkina, Ekaterina, Mangos, Maria M, Elzagheid, Mohamed I, Damha, Masad J
Format Journal Article
LanguageEnglish
Published United States 01.11.2002
Subjects
Arabinonucleotides - chemical synthesis
Arabinose - analogs & derivatives
Oligonucleotides - chemical synthesis
Phosphorothioate Oligonucleotides - chemical synthesis
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Summary:This unit describes the chemical synthesis of 2'-deoxy-2'-fluoro-b-D-oligoarabinonucleotides (2'F-ANA), both with phosphodiester and phosphorothioate linkages. The protocols described herein include araF phosphoramidite preparation, assembly on DNA synthesizers, and final deprotection and purification of oligonucleotides.
ISSN:1934-9289
DOI:10.1002/0471142700.nc0415s10