TGF-β1-induced Synthesis of Collagen Fibers in Skeletal Muscle-Derived Stem Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 33; no. 2; pp. 238 - 243
• Main Author: 陈燕花 彭云龙 王旸 翁雨雄 李涛 张燕 陈振兵
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.04.2013; Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
• Subjects: Animals; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cells, Cultured; CTGF; Fibrillar Collagens - biosynthesis; Male; Medicine; Medicine & Public Health; mRNA水平; Myoblasts, Skeletal - cytology; Myoblasts, Skeletal - drug effects; Myoblasts, Skeletal - metabolism; Rats; Rats, Sprague-Dawley; RT-PCR; Stem Cells - cytology; Stem Cells - drug effects; Stem Cells - metabolism; Transforming Growth Factor beta1 - pharmacology; 细胞合成; 结缔组织生长因子; 胶原纤维; 诱导; 骨骼肌; muscle-derived stem cells; TGF-β1; CTGF; TGF-β 1
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-013-1104-0

The aim of this study was to investigate the mechanism of deposition of extracellular matrix induced by TGF-β1 in skeletal muscle-derived stem cells （MDSCs）. Rat skeletal MDSCs were obtained by using preplate technique, and divided into four groups： group A （control group）, group B （treated with TGF-β1, 10 ng/rnL）, group C （treated with TGF-β1 and anti-connective tissue growth factor （CTGF）, both in 10 ng/mL）, and group D （treated with anti-CTGF, 10 ng/mL）. The expression of CTGF, collagen type- I （COL- I ） and collagen type-III （COL-III） in MDSCs was examined by using RT-PCR, Western blot and immunofluorescent stain. It was found that one day after TGF-β1 treatment, the expression of CTGF, COL- I and COL-Ⅲ was increased dramatically. CTGF expression reached the peak on the day 2, and then decreased rapidly to a level of control group on the day 5. COL- I and COL-Ⅲ mRNA levels were overexpresed on the day 2 and 3 respectively, while their protein expression levels were up-regulated on the day 2 and reached the peak on the day 7. In group C, anti-CTGF could partly suppress the overexpression of COL-I and COL-Ill induced by TGF-131 one day after adding CTGF antibody. It was concluded that TGF-β1 could induce MDSCs to express CTGF, and promote the production of COL- I and COL-III. In contrast, CTGF antibody could partially inhibit the effect of TGF-β1 on the MDSCs by reducing the expression of COL- I and COL-III. Taken together, we demonstrated that TGF-β1-CTGF signaling played a crucial role in MDSCs synthesizing collagen proteins in vitro, which provided theoretical basis for exploring the methods postponing skeletal muscle fibrosis after nerve injury.