Random primer dependent PCR differentiates aggressive from non-aggressive isolates of the oilseed rape pathogen Phoma lingam (Leptosphaeria maculans)

• Published in: Journal of phytopathology Vol. 136; no. 2; pp. 124 - 136
• Main Authors: Schaefer, C. (Institut fuer Genbiologische Forschung Berlin G.m.b.H. (Germany)), Wostemeyer, J
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.10.1992; Blackwell
• Subjects: Agronomy. Soil science and plant productions; Biol ogie; BIOLOGIA MOLECULAR; Biological and medical sciences; BIOLOGIE MOLECULAIRE; BRASSICA NAPUS; Chemie; Chromatographie; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Fungi; IDENTIFICACION; IDENTIFICATION; Identifizierung; LEPTOSPHAERIA MACULANS; Oelpflanze; PATHOTYPE; PATOTIPOS; PHOMA; Phoma lingam; Phytopathology. Animal pests. Plant and forest protection; Rasse; Variation, races, biotypes, parasitic specialization, genetics; Brassica napus var. oleifera; Genetic marker; Mycosis; Plant pathogen; Fungi; Infection; Phoma lingam; Polymerase chain reaction; Leptosphaeria maculans; Cruciferae; Dicotyledones; DNA; Pathotype; Angiospermae; RAPD markers; Isolate; Ascomycetes; Spermatophyta; Oligonucleotide; Fungi Imperfecti; Thallophyta; Aggressiveness
• ISSN: 0931-1785; 1439-0434
• DOI: 10.1111/j.1439-0434.1992.tb01290.x

A number of isolates of the aggressive and non-aggressive pathotype group of the rape seed (Brassica napus) pathogen Phoma lingam (teleomorph: Leptosphaeria maculans) were analyzed by means of a simple, random primer dependent (PCR (polymerase chain reaction) approach. Using the four synthetic nona- and decamer oligonucleotides 5-GGAGCCCAC-3, 5''-ACGGTCTTGG-3', 5'GAAACAGCGG-3', and 5'-GGCATCGGCC-3' informative bands for both of the pathotype groups could be obtained. These amplified bands were shown to originate not only from repeated but also from single and low copy target sequences. This is the first report on molecular diagnostic of a plant pathogenic fungus, based on random primer dependent PCR. The experimental system is fast and reliable, does not require cloning and sequencing of L. maculans DNA, and works without time-consuming blotting or hybridization steps