Fungal Pathogen Emergence: Investigations with an Ustilago   maydis × Sporisorium reilianum Hybrid

• Published in: Journal of fungi (Basel) Vol. 7; no. 8; p. 672
• Main Authors: Storfie, Emilee R M, Saville, Barry J
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.08.2021; MDPI
• Subjects: Anthocyanins; Colonization; Crop production; Dikaryons; Filaments; fungal hybridization; Fungi; Gene expression; Genomes; Hybridization; Infections; Pathogenesis; pathogenesis assays; Pathogens; Smut; Sporisorium reilianum; Transcription factors; Tumors; Ustilago maydis; Virulence; virulence factors; Canada; United States > US; Ustilago maydis; transcription factors; pathogenesis assays; effectors; Sporisorium reilianum; fungal hybridization; virulence factors
• ISSN: 2309-608X; 2309-608X
• DOI: 10.3390/jof7080672

The emergence of new fungal pathogens threatens sustainable crop production worldwide. One mechanism by which new pathogens may arise is hybridization. To investigate hybridization, the related smut fungi and , were selected because they both infect , can hybridize, and tools are available for their analysis. The hybrid dikaryons of these fungi grew as filaments on plates but their colonization and virulence in were reduced compared to the parental dikaryons. The anthocyanin induction caused by the hybrid dikaryon infections was distinct, suggesting its interaction with the host was different from that of the parental dikaryons. Selected virulence genes previously characterized in and their predicted orthologs had altered transcript levels during hybrid infection of . The downregulated effectors, and and transcription factors, , , and were constitutively expressed in the hybrid. Little impact was observed with increased effector expression; however, increased expression of and which regulate early pathogenic development by , increased the hybrid's capacity to induce symptoms including the rare induction of small leaf tumors. These results establish a base for investigating molecular aspects of smut fungal hybrid pathogen emergence.