Use of silica gel with reversed-phase eluents for the separation and determination of alkaloids in Corydalis Yanhusuo W. T. Wang and its preparations

An HPLC method using unmodified silica with reversed‐phase eluents was developed for the determination of DL‐tetrahydropalmatine in Corydalis Yanhusuo W. T. Wang and its set prescription preparations. The mobile phase was a mixture of methanol and 2 mmol/L phosphate buffer (75:25, v/v). The result w...

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Bibliographic Details
Published inBiomedical chromatography Vol. 10; no. 1; pp. 11 - 14
Main Authors Yuan, Y F, Liu, Z L, Li, X L
Format Journal Article
LanguageEnglish
Published Chichester, UK John Wiley & Sons, Ltd 01.01.1996
Subjects
Berberine Alkaloids - analysis
Chromatography, High Pressure Liquid - methods
Chromatography, High Pressure Liquid - statistics & numerical data
Drugs, Chinese Herbal - analysis
Hydrogen-Ion Concentration
Sensitivity and Specificity
Silica Gel
Silicon Dioxide
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Summary:An HPLC method using unmodified silica with reversed‐phase eluents was developed for the determination of DL‐tetrahydropalmatine in Corydalis Yanhusuo W. T. Wang and its set prescription preparations. The mobile phase was a mixture of methanol and 2 mmol/L phosphate buffer (75:25, v/v). The result was calculated by external standardization. The method is simple, rapid and precise, and could also be used for the separation and determination of alkaloids in other crude drugs and preparations.
Bibliography:istex:0D2EFC3B3460251EFE50E57E63FF2B9C29CA435B
ark:/67375/WNG-F8DH8KN4-1
ArticleID:BMC541
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0269-3879
1099-0801
DOI:10.1002/(SICI)1099-0801(199601)10:1<11::AID-BMC541>3.0.CO;2-Q