Low temperature embedding with Lowicryl resins: two new formulations and some applications

Lowicryl K4M and HM20 are methacrylate/acrylate based low temperature embedding resins for biological material which can be used in conjunction with either the progressive lowering of temperature (PLT) technique or with freeze-substitution. K4M and HM20 are applicable over a very extended temperatur...

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Bibliographic Details
Published inJournal of microscopy (Oxford) Vol. 140; no. Pt 1; p. 55
Main Authors Carlemalm, E, Villiger, W, Hobot, J A, Acetarin, J D, Kellenberger, E
Format Journal Article
LanguageEnglish
Published England 01.10.1985
Subjects
Acrylic Resins
Animals
Escherichia coli - analysis
Freeze Drying - methods
Microscopy, Electron - methods
Microtomy - methods
Polymers
Rats
Temperature
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Summary:Lowicryl K4M and HM20 are methacrylate/acrylate based low temperature embedding resins for biological material which can be used in conjunction with either the progressive lowering of temperature (PLT) technique or with freeze-substitution. K4M and HM20 are applicable over a very extended temperature range, approximately 220 K to 340 K. With two new resins, K11M and HM23, one can reach even lower temperatures, c. 200 K. Freeze-substitution combined with low temperature embedding allows for very mild or no chemical fixation which seems to increase the sensitivity of immunocytochemical localization of antigens on sections.
ISSN:0022-2720
DOI:10.1111/j.1365-2818.1985.tb02660.x