Effect of Strong Light on Chlorophyll Degradation in Strawberry Leaves

• Published in: Russian journal of plant physiology Vol. 69; no. 7
• Main Authors: Luo, T. Q., Zhao, W. Z., Zhang, Y. Z., Xu, S. R., Xiao, W., Li, L.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.12.2022; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Chlorophyll; Degradation; Environmental degradation; Fragaria ananassa; Fruits; Genes; Life Sciences; Light; Oxygenase; Phaeophytin; Photodegradation; Plant breeding; Plant Physiology; Plant Sciences; Red chlorophyll catabolite reductase; Reductases; Research Papers; Seedlings; Strawberries; Sunburn; strawberry; chlorophyll degradation; strong light stress; ×
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443722601483

Strawberry seedlings are often subjected to strong light stress during breeding and strawberry production, leading to seedling death, sunburn and disease. We investigated the endogenous mechanism of strong light stress in strawberry by studying chlorophyll degradation. The activity of chlorophyll, intermediate products, chlorophyll degradation key enzymes and the expression of chlorophyll degradation related genes were measured in ‘Xuelixiang’ strawberry ( Fragaria × ananassa Duch.) under 12 hours of 30 000 xl and 100 000 xl light treatment respectively. It was found that the concentrations of chlorophyll, chlorophyll a and chlorophyll b in strawberry leaves decreased significantly and the ratio of chlorophyll a to b increased under strong light stress treatment; pheophytin pheophorbide hydrolase (PPH), pheophorbide a oxygenase (PAO) and red chlorophyll catabolite reductase (RCCR) activities were higher than those in the NL group; chlorophyll degradation-related genes (except FaMDCase ) were all higher expressed than in the NL group. The degradation of strawberry chlorophyll was accelerated by strong light stress. In addition, showing that PPH and PAO are key factors in the chlorophyll degradation pathway of strawberry. This study lays the foundation for an in-depth study on chlorophyll degradation in strawberry under strong light stress and other related studies, aiming to provide a theoretical basis for light management during strawberry production.