Isolation and characterization of two distinct Class II PR4 genes from the oriental lily hybrid Sorbonne
Pathogenesis-related (PR) proteins are generally involved in the defense of plants and are important contributors in the disease resistance of plants. Among the 17 PRs that are currently recognized, the PR4 family of proteins is divided into two classes and features a conserved barwin domain. In thi...
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Published in | Russian journal of plant physiology Vol. 64; no. 5; pp. 707 - 717 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Moscow
Pleiades Publishing
01.09.2017
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Pathogenesis-related (PR) proteins are generally involved in the defense of plants and are important contributors in the disease resistance of plants. Among the 17 PRs that are currently recognized, the PR4 family of proteins is divided into two classes and features a conserved barwin domain. In this study, we isolated two Class II
PR4s
from the oriental hybrid lily cultivar Sorbonne using the rapid amplification of the cDNA ends (RACE) method, and designated these two PR4s
LhSorPR4a
and
LhSorPR4b
.
LhSorPR4a
and
LhSorPR4b
were 627 and 617 bp in length, respectively, and encoded two corresponding PR4s of 141 and 143 amino acids. These deciphered LhSorPR4a and LhSorPR4b protein sequences shared a sequence similarity of 90.7%, but their theoretical isoelectric points were distinctively different (7.74 and 4.08, respectively). The three-dimensional structures of LhSorPR4a and LhSorPR4b predicted by homology modeling showed high similarity to their corresponding papaya barwin-like protein template. Analysis of expression by qPCR revealed that both
LhSorPR4a
and
LhSorPR4b
were responsive to methyl jasmonate and ethephon treatments. The
LhSorPR4b
expression was also significantly induced by sodium salicylate (SS); however,
LhSorPR4a
was unresponsive to the SS treatment. Both LhSorPR4a and LhSorPR4b were expressed in
Escherichia coli
(
E. coli
) and successfully purified. The
PR4s
characterized in this study (
LhSorPR4a
and
LhSorPR4b
) are the first two
PR4
family genes isolated from the
Lilium
genus, and they could therefore play an important role in lily disease resistance. |
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ISSN: | 1021-4437 1608-3407 |
DOI: | 10.1134/S1021443717050132 |