Extraction and Quantification of Abscisic Acid and Derivatives in Strawberry by LC-MS

• Published in: Food analytical methods Vol. 11; no. 9; pp. 2547 - 2552
• Main Authors: Perin, Ellen Cristina, Crizel, Rosane Lopes, Galli, Vanessa, da Silva Messias, Rafael, Rombaldi, Cesar Valmor, Chaves, Fabio Clasen
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.09.2018; Springer Nature B.V
• Subjects: Abscisic acid; Acetic acid; Acetone; Analytical Chemistry; Berries; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Derivatives; Food Science; Fruits; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Microbiology; Sonication; Abscisic acid; Phaseic acid; Liquid chromatography-mass spectrometry; Abscisic acid glucose ester; Dihydrophaseic acid
• ISSN: 1936-9751; 1936-976X
• DOI: 10.1007/s12161-018-1224-z

Phytohormones are important plant components that are involved in a signaling cascade in plant development. In strawberry, the influence of abscisic acid (ABA) and its different forms [phaseic acid (PA), dihydrophaseic acid (DPA), and ABA glucose ester (ABA-GE)] on the process of fruit maturation is not yet completely understood. Quantification of phytohormones is currently performed by liquid chromatography coupled to mass spectrometry (LC-MS) due to its sensitivity and specificity. However, the sample matrix and the extraction procedure will influence the analysis. Thus, this study aimed to optimize a simple extraction method and validate the LC-MS quantification of ABA as well as the identification and quantification of ABA derivatives (PA, DPA, and ABA-GE) in strawberry fruit. Hormone extraction was performed using either methanol (80% v / v —S1) or acetone:water:acetic acid (80:19:1 v / v —S2) solutions with or without the use of sonication. The most efficient extraction was obtained using S1 without sonication, and LC-MS validation parameters for ABA were within acceptable scores.