Determination of Nitrofuran Metabolites in Muscular Tissue by High-Performance Liquid Chromatography with Mass Spectrometric Detection

We developed a procedure for determining residual amounts of four nitrofuran metabolites (3‑amino-2-oxazolidinone, 3-amino-5-methylmorpholino-2-oxazolidinone, 1-aminohydantoin hydrochloride, and semicarbazide hydrochloride) in muscle tissue in the form of 2-nitrophenyl derivatives by HPLC with selec...

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Bibliographic Details
Published inJournal of analytical chemistry (New York, N.Y.) Vol. 74; no. 9; pp. 906 - 912
Main Authors Kulikovskii, A. V., Gorlov, I. F., Slozhenkina, M. I., Vostrikova, N. L., Ivankin, A. N., Kuznetsova, O. A.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.09.2019
Springer Nature B.V
Subjects
Analytical Chemistry
Chemistry
Chemistry and Materials Science
High performance liquid chromatography
Ion migration
Ions
Metabolites
Muscles
Selectivity
Spectrometry
nitrofuran metabolites
1-aminohydantoin (AHD)
HPLC with mass spectrometric detection
3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ)
semicarbazide (SEM)
3-amino-2-oxazolidinone (AOZ)
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Summary:We developed a procedure for determining residual amounts of four nitrofuran metabolites (3‑amino-2-oxazolidinone, 3-amino-5-methylmorpholino-2-oxazolidinone, 1-aminohydantoin hydrochloride, and semicarbazide hydrochloride) in muscle tissue in the form of 2-nitrophenyl derivatives by HPLC with selected reaction monitoring mass spectrometric detection. The effect of the matrix suppression of ionization is shown. The selectivity of extraction is increased by optimizing the sample preparation procedure, and the effect of organic impurities on the result measurements is minimized. The lower limit of the analytical range is 10 μg/kg for semicarbazide hydrochloride and 1 μg/kg for the remaining nitrofuran metabolites.
ISSN:1061-9348
1608-3199
DOI:10.1134/S106193481907013X