Molecular Mechanisms Endowing Cross-resistance to ALS-Inhibiting Herbicides in Amaranthus hybridus from Argentina

• Published in: Plant molecular biology reporter Vol. 36; no. 5-6; pp. 907 - 912
• Main Authors: Larran, Alvaro S., Lorenzetti, Florencia, Tuesca, Daniel, Perotti, Valeria E., Permingeat, Hugo R.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2018; Springer Nature B.V
• Subjects: Acetolactate synthase; Amaranthus hybridus; Amino acid sequence; Amino acids; Bioinformatics; Biomedical and Life Sciences; Cross-resistance; Herbicides; Life Sciences; Metabolomics; Molecular modelling; Plant Breeding/Biotechnology; Plant Sciences; Populations; Proteomics; Short Communication; Argentina; Acetolactate synthase; Weed control; Herbicide resistance; Weeds
• ISSN: 0735-9640; 1572-9818
• DOI: 10.1007/s11105-018-1122-y

Amaranthus hybridus L. is one of the most problematic weeds in summer crops in Argentina. However, 20 years after the detection of the first case of resistance to ALS-inhibiting herbicides in this country, no extensive reports of the molecular mechanisms endowing resistance were published. In this work, we sequenced the acetolactate synthase gene of resistant plants belonging to five different populations of A. hybridus from Santa Fe and Cordoba provinces. We found that every population presented at least one of the previously documented substitutions W574L and D376E in ALS amino acid sequence. These results explain the cross-resistance to ALS-inhibiting herbicides and should alert about the usage of herbicides with a different site of action after an ineffective control of this species. This is the first report of these target-site mechanisms endowing resistance to ALS-inhibiting herbicides in A. hybridus populations from Argentina.