Gene Expression Analysis as a Method to Predict OTA Accumulation in Dry-Cured Meat Products
Dry-cured meat products are frequently colonised by toxigenic Penicillium nordicum and Penicillium verrucosum throughout their ripening that can produce ochratoxin A (OTA). The predictive nature of the molecular analyses can be used to determine the risk of toxigenic species. For this reason, the ob...
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Published in | Food analytical methods Vol. 11; no. 9; pp. 2463 - 2471 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.09.2018
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Dry-cured meat products are frequently colonised by toxigenic
Penicillium nordicum
and
Penicillium verrucosum
throughout their ripening that can produce ochratoxin A (OTA). The predictive nature of the molecular analyses can be used to determine the risk of toxigenic species. For this reason, the objective of the present work was to analyse the temporal changes in the expression of the
otapks
and
otanps
genes by
P. nordicum
and
P. verrucosum
in relation to OTA production on slices of dry-fermented sausage
salchichón
and dry-cured ham. Gene expression was higher in
P. nordicum
than in
P. verrucosum
in both meat matrices. The
otapks
gene was overexpressed by both
Penicillium
species, especially in
salchichón
. OTA was only detected in inoculated
salchichón
regardless of the ochratoxigenic species used. The high significant correlation found between the early relative expression of the
otapks
gene and OTA production in
salchichón
leads to propose the relative gene expression of the
otapks
gene as a good indicator to predict OTA accumulation throughout the ripening of this product. |
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ISSN: | 1936-9751 1936-976X |
DOI: | 10.1007/s12161-018-1231-0 |