Posttranscriptional regulation of the T-box gene midline via the 3′UTR in Drosophila is complex and cell- and tissue-dependent

• Published in: Genetics (Austin) Vol. 227; no. 4
• Main Authors: Makhijani, Kalpana, Mar, Jordan, Gaziova, Ivana, Bhat, Krishna Moorthi
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 01.08.2024; Genetics Society of America
• Subjects: 3' Untranslated regions; Amino acids; Degradation; Drosophila; Ectopic expression; Fruit flies; Gene expression; Gene sequencing; Insects; Investigation; mRNA; Post-transcription; Proteins; Ribonucleic acid; RNA; T-box gene; Transgenes; Translation; Wings; experimental technology; genetics; transcription; cell culture; gene; Drosophila; 3′UTR
• ISSN: 1943-2631; 0016-6731; 1943-2631
• DOI: 10.1093/genetics/iyae087

Abstract The T-box (Tbx) proteins have a 180–230 amino acid DNA-binding domain, first reported in the Brachyury (T) protein. They are highly conserved among metazoans. They regulate a multitude of cellular functions in development and disease. Here, we report posttranscriptional and translational regulation of midline (mid), a Tbx member in Drosophila. We found that the 3′UTR of mid has mRNA degradation elements and AT-rich sequences. In Schneider S2 cells, mid-mRNA could be detected only when the transgene was without the 3′UTR. Similarly, the 3′UTR linked to the Renilla luciferase reporter significantly reduced the activity of the Luciferase, whereas deleting only the degradation elements from the 3′UTR resulted in reduced activity, but not as much. Overexpression of mid in MP2, an embryonic neuroblast, showed no significant difference in the levels of mid-mRNA between the 2 transgenes, with and without the 3′UTR, indicating the absence of posttranscriptional regulation of mid in MP2. Moreover, while elevated mid-RNA was detected in MP2 in nearly all hemisegments, only a fifth of those hemisegments had elevated levels of the protein. Overexpression of the 2 transgenes resulted in MP2-lineage defects at about the same frequency. These results indicate a translational/posttranslational regulation of mid in MP2. The regulation of ectopically expressed mid in the wing imaginal disc was complex. In the wing disc, where mid is not expressed, the ectopic expression of the transgene lacking the 3′UTR had a higher level of mid-RNA and the protein had a stronger phenotypic effect. These results indicate that the 3′UTR can subject mid-mRNA to degradation in a cell- and tissue-specific manner. We further report a balancer-mediated transgenerational modifier effect on the expression and gain of function effects of the 2 transgenes. Graphical Abstract Graphical Abstract