Preparation of Cerium Dioxide Functionalized Magnetic Layered Double Hydroxides for High-efficiency Phosphopeptide Enrichment

• Published in: Chemical research in Chinese universities Vol. 39; no. 2; pp. 253 - 259
• Main Authors: Jiang, Dandan, Qi, Ruixue, Lyu, Siqi, Wang, Wei, Liu, Jinghai, Jia, Qiong
• Format: Journal Article
• Language: English
• Published: Changchun Jilin University and The Editorial Department of Chemical Research in Chinese Universities 01.04.2023; Springer Nature B.V
• Subjects: Affinity; Analytical Chemistry; Biological properties; Casein; Cerium oxides; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chromatography; Dioxides; Hydroxides; Inorganic Chemistry; Iron oxides; Metal oxides; Nanoparticles; Organic Chemistry; Physical Chemistry; Magnetic; Layered double hydroxide; Cerium dioxide; Phosphopeptide enrichment
• ISSN: 1005-9040; 2210-3171
• DOI: 10.1007/s40242-022-2165-z

I n this work, we prepared a material with magnetic nanoparticles (Fe 3 O 4 ) as core, layered double hydroxides(LDHs) as affinity shell, and cerium dioxide(CeO 2 ) as functional molecules(denoted as Fe 3 O 4 @LDH-CeO 2 ). On the basis of combined immobilized metal ion affinity chromatography(IMAC) and metal oxide affinity chromatography(MOAC), Fe 3 O 4 @LDH-CeO 2 was used to enrich phosphopeptides with high efficiency. The material exhibited high selectivity( α -casein: β -casein:BSA=1:1:5000, mass ratio), high recovery(95.87%), and good reusability of 10 times adsorption-desorption experiments. The feasibility of Fe 3 O 4 @LDH-CeO 2 was further investigated by extracting phosphopeptides from biological samples(nonfat milk, serum, saliva, and A549 cell lysate).