Isolation and characterization of eleven novel polymorphic microsatellite loci in the spiny softshell turtle (Apalone spinifera)

We isolate and characterize 11 microsatellite loci for the spiny softshell turtle ( Apalone spinifera) from a partial genomic library obtained using 454 sequencing technology . Genotypes of 15 individuals from southern Ontario and 30 individuals of unknown origin contain 6–20 alleles per locus and t...

Full description

Saved in:
Bibliographic Details
Published inConservation genetics resources Vol. 4; no. 3; pp. 759 - 761
Main Authors Davy, Christina M., Conflitti, Ida M., Storisteanu, Daniel M. L., Murphy, Robert W.
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.09.2012
Springer Nature B.V
Subjects
Animal Genetics and Genomics
Apalone spinifera
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Genotypes
Heterozygosity
Life Sciences
Plant Genetics and Genomics
Population genetics
Population studies
Technical Note
Microsatellite
Turtle
Next-generation sequencing
Online AccessGet full text

Cover

More Information
Summary:We isolate and characterize 11 microsatellite loci for the spiny softshell turtle ( Apalone spinifera) from a partial genomic library obtained using 454 sequencing technology . Genotypes of 15 individuals from southern Ontario and 30 individuals of unknown origin contain 6–20 alleles per locus and the level of heterozygosity ranges from 0.229 to 0.800. These loci will be useful for population genetics studies and enforcement activities such as assignment of illegally traded individuals to their population of origin.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-012-9638-1